Long-Term Storage Does Not Affect the Expression Profiles of mRNA and Long Non-Coding RNA in Vitrified-Warmed Human Embryos.
Long-Term Storage Does Not Affect the Expression Profiles of mRNA and Long Non-Coding RNA in Vitrified-Warmed Human Embryos.
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DOI：
10.3389/fgene.2021.751467
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发表时间：
2021
影响因子：
3.7
通讯作者：
Huang G
中科院分区：
文献类型：
--
作者：
Li J;Zhu L;Huang J;Liu W;Han W;Huang G
尽管玻璃化冷冻已广泛应用于辅助生殖技术，但保存时间是否对人类胚胎中的mRNA和lncRNA表达谱有影响尚不清楚。本研究招募了 11 名接受过体外受精治疗的女性（年龄 23-35 岁）。通过单细胞 RNA-Seq 分析了 3 个新鲜八细胞胚胎和 8 个存活的玻璃化加热八细胞胚胎（其中 4 个胚胎冷冻保存 3 年，其他胚胎冷冻保存 8 年）的转录组。 3岁组和8岁组之间未发现差异表达的mRNA或lncRNA。与新鲜胚胎相比，8 个玻璃化加热胚胎中共有 128 个 mRNA 和 365 个 lncRNA 存在差异表达。玻璃化升温影响中等，主要与代谢、应激反应、细胞凋亡、细胞周期、细胞粘附以及TFG-β和Hippo信号通路有关。对目标 mRNA 的分析表明，lncRNA 可能有助于玻璃化升温后 mRNA 的调节。我们的研究结果表明，玻璃化后的长期储存不会影响人类胚胎中的mRNA和lncRNA表达谱，然而，玻璃化升温过程会导致转录组的微小改变。
Although vitrification has been widely applied in assisted reproductive technology, it is unknown whether storage time has any impact on the mRNA and lncRNA expression profiles in human embryos. Eleven women (aged 23–35 years) who had undergone in vitro fertilization treatment were recruited for this study. The transcriptomes of 3 fresh eight-cell embryos and 8 surviving vitrified-warmed eight-cell embryos (4 embryos were cryostored for 3 years, and the others were cryostored for 8 years) were analyzed through single-cell RNA-Seq. No differentially expressed mRNAs or lncRNAs were identified between the 3-years group and 8-years group. A total of 128 mRNAs and 365 lncRNAs were differentially expressed in the 8 vitrified-warmed embryos compared with the fresh embryos. The vitrification-warming impact was moderate, and it was mainly related to the pathways of metabolism, stress response, apoptosis, cell cycle, cell adhesion, and signaling for TFG-β and Hippo. The analysis of target mRNAs suggested that lncRNAs might contribute to the regulation of mRNAs after vitrification-warming. Our findings indicated that long-term storage after vitrification does not affect the mRNA and lncRNA expression profiles in human embryos, however, the procedure of vitrification-warming would lead to minor alteration of transcriptome.
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DOI：
10.1007/bf01131376
发表时间：
1986-01-01
期刊：
Journal of In Vitro Fertilization and Embryo Transfer
影响因子：
--
作者：
GLENISTER P H;LYON M F
通讯作者：
LYON M F
影响因子：
2.7
作者：
Li, Jingyu;Huang, Jiayu;Huang, Guoning
通讯作者：
Huang, Guoning
影响因子：
8.1
作者：
Huo, Ying;Yuan, Peng;Qiao, Jie
通讯作者：
Qiao, Jie
影响因子：
2.5
作者：
Aksu, Digdem Aktoprakligil;Agca, Cansu;Agca, Yuksel
通讯作者：
Agca, Yuksel
DOI：
10.1242/dev.116996
发表时间：
2015-03-01
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Hamazaki N;Uesaka M;Nakashima K;Agata K;Imamura T
通讯作者：
Imamura T