Reversible Ratiometric NADH Sensing Using Semiconducting Polymer Dots.
Reversible Ratiometric NADH Sensing Using Semiconducting Polymer Dots.
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DOI：
10.1002/anie.202100774
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发表时间：
2021-05-17
期刊：
影响因子：
--
通讯作者：
Chiu DT
中科院分区：
文献类型：
--
作者：
Chen H;Yu J;Men X;Zhang J;Ding Z;Jiang Y;Wu C;Chiu DT
还原型烟酰胺腺嘌呤二核苷酸（reduced nicotinamide adenine dinucleotide，NADH）是活细胞中的关键辅酶，在氧化还原反应中起电子载体的作用，其浓度是细胞代谢状态的重要指标。异常的NADH水平与年龄相关的代谢疾病和神经退行性疾病有关，这就需要一种简单、快速的即时检测NADH的分析方法。在这里，我们开发了一系列的NADH敏感的半导体聚合物点（Pdots）作为纳米探针的NADH测量，并测试其性能在体外和体内。NADH感测基于在UV激发时从Pdot中的半导体聚合物链到NADH的电子转移，猝灭Pdot荧光发射。在基于聚芴的Pdot中，这种机制导致了开关型NADH传感器;在DPA-CNPPV Pdot中，UV激发导致了在两个波长处的NADH敏感发射，使得能够进行比率检测。使用DPA-CNPPV Pdots的比率型NADH检测具有高灵敏度（检测限为3.1 μM）、相对于其他分析物的优异选择性、可逆性和快速响应（小于5 s）。我们展示了比率NADH传感Pdot的应用，包括基于智能手机的NADH成像，用于即时护理。一种简单、快速的NADH传感分析方法是通过在紫外激发下从Pdot到NADH的电子转移来实现的。使用DPA-CNPPV Pdots的比率式NADH检测具有高灵敏度、相对于其他分析物的优异选择性以及快速和可逆的响应。进行基于智能手机的NADH体内比率成像以证明床旁使用的可行性。
Reduced nicotinamide adenine dinucleotide (NADH) is a key coenzyme in living cells due to its role as an electron carrier in redox reactions, and its concentration is an important indicator of cell metabolic state. Abnormal NADH levels are associated with age-related metabolic diseases and neurodegenerative disorders, creating a demand for a simple, rapid analytical method for point-of-care NADH sensing. Here we develop a series of NADH-sensitive semiconducting polymer dots (Pdots) as nanoprobes for NADH measurement, and test their performance in vitro and in vivo. NADH sensing is based on electron transfer from semiconducting polymer chains in the Pdot to NADH upon UV excitation, quenching Pdot fluorescence emission. In polyfluorene-based Pdots, this mechanism resulted in an on-off NADH sensor; in DPA-CNPPV Pdots, UV excitation resulted in NADH-sensitive emission at two wavelengths, enabling ratiometric detection. Ratiometric NADH detection using DPA-CNPPV Pdots exhibits high sensitivity (3.1 μM limit of detection), excellent selectivity versus other analytes, reversibility, and a fast response (less than 5 s). We demonstrate applications of the ratiometric NADH-sensing Pdots including smartphone-based NADH imaging for point-of-care use. A simple, rapid analytical method for NADH sensing is achieved by electron transfer from a Pdot to NADH upon UV excitation. Ratiometric NADH detection using DPA-CNPPV Pdots exhibits high sensitivity, excellent selectivity versus other analytes, and a fast and reversible response. Smartphone-based in vivo ratiometric imaging of NADH is performed to demonstrate the feasibility of point-of-care use.
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