Characterization of bacterial biota in the distal esophagus of Japanese patients with reflux esophagitis and Barrett's esophagus.
Characterization of bacterial biota in the distal esophagus of Japanese patients with reflux esophagitis and Barrett's esophagus.
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DOI：
10.1186/1471-2334-13-130
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发表时间：
2013-03-11
影响因子：
3.7
通讯作者：
Goto H
中科院分区：
文献类型：
--
作者：
Liu N;Ando T;Ishiguro K;Maeda O;Watanabe O;Funasaka K;Nakamura M;Miyahara R;Ohmiya N;Goto H
远端食管含有复杂的细菌群。我们假设更好地了解食管中的细菌群落将有助于了解细菌在食管疾病中的作用。在这里，我们研究了正常食管、反流性食管炎和巴雷特食管受试者远端食管的细菌组成。内镜下取18例患者（正常食管、反流性食管炎、Barrett食管各6例）远端食管胃食管交界处上方1cm处活检标本2份，用于组织学检查和DNA提取。使用通用细菌引物通过 PCR 扩增 16S rDNA 基因片段，并检查细菌群体。第三份活检标本取自 Barrett 食管患者，以在组织学上证实远端食管中鳞状上皮被柱状上皮替代。正常食管、食管炎和巴雷特食管的内镜诊断均通过组织学检查结果得到证实。检测到的细菌 DNA 总量在各组之间没有显着差异 (p > 0.1)。平均而言，18 名受试者每人产生约 350 个克隆，其中 40 个是随机挑选并测序的。对 6 名正常食管受试者的 240 个克隆的 147 个 16S rDNA 序列进行分析，得出四个门：变形菌门 (49%)、厚壁菌门 (40%)、拟杆菌门 (8%) 和放线菌门 (3%)。对 6 名反流性食管炎患者的 240 个克隆的 139 个 16S rDNA 序列进行类似分析，得出 6 个门：变形菌门 (43%)、厚壁菌门 (33%)、拟杆菌门 (10%)、梭杆菌门 (10%)、放线菌门 (2%) 和 TM7 (2%)。 6例Barrett食管的240个克隆的138个16S rDNA序列产生了5个门，厚壁菌门（55%）、变形菌门（20%）、拟杆菌门（14%）、梭杆菌门（9%）和放线菌门（2%）。因此，正常食管、反流性食管炎和巴雷特食管患者的微生物群落有所不同。正常食管、反流性食管炎和巴雷特食管的食管细菌组成有所不同。不同的细菌群落可能与食管疾病有关。
The distal esophagus harbors a complex bacterial population. We hypothesized that a better understanding of bacterial communities in the esophagus would facilitate understanding of the role of bacteria in esophageal disease. Here, we investigated bacterial composition in the distal esophagus in subjects with a normal esophagus, reflux esophagitis, and Barrett’s esophagus. Two biopsy specimens were obtained from the distal esophagus at 1 cm above the gastroesophageal junction under endoscopic examination in 18 patients (6 each with normal esophagus, reflux esophagitis, and Barrett’s esophagus) and used for histological examination and DNA extraction. Fragments of 16S rDNA genes were amplified by PCR using general bacterial primers, and bacterial populations were examined. A third biopsy specimen was taken from the patients with Barrett’s esophagus to histologically confirm the replacement of squamous epithelium with columnar epithelium in the distal esophagus. Endoscopic diagnoses of normal esophagus, esophagitis, and Barrett’s esophagus were confirmed by histological findings. The total amount of bacterial DNA detected did not significantly differ among groups (p > 0.1). On average, each of the 18 subjects yielded about 350 clones, of which 40 were randomly picked and sequenced. Analysis of 147 16S rDNA sequences from 240 clones of 6 subjects with normal esophagus yielded four phyla, Proteobacteria (49%), Firmicutes (40%), Bacteroidetes (8%), and Actinobacteria (3%). Similar analysis of 139 16S rDNA sequences from 240 clones of 6 patients with reflux esophagitis yielded 6 phyla, Proteobacteria (43%), Firmicutes (33%), Bacteroidetes (10%), Fusobacteria (10%), Actinobacteria (2%), and TM7 (2%). while that of 138 16S rDNA sequences from 240 clones of 6 cases of Barrett’s esophagus yielded 5 phyla, Firmicutes (55%), Proteobacteria (20%), Bacteroidetes (14%), Fusobacteria (9%), and Actinobacteria (2%). Thus, microbial communities differed among patients with a normal esophagus, reflux esophagitis and Barrett’s esophagus. Esophageal bacterial composition differs under conditions of normal esophagus, reflux esophagitis, and Barrett’s esophagus. Diverse bacterial communities may be associated with esophageal disease.
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影响因子：
29.4
作者：
Spechler, Stuart J.;Sharma, Prateek;Yao, John
通讯作者：
Yao, John
影响因子：
9.8
作者：
Ho, KY;Chan, YH;Kang, JY
通讯作者：
Kang, JY
影响因子：
3.1
作者：
Ashktorab, Hassan;Entezari, Omid;Razjouyan, Hadie
通讯作者：
Razjouyan, Hadie
DOI：
10.1093/jnci/djn211
发表时间：
2008-08-20
期刊：
Journal of the National Cancer Institute
影响因子：
--
作者：
Brown LM;Devesa SS;Chow WH
通讯作者：
Chow WH
影响因子：
2.6
作者：
Hayashi, H;Sakamoto, M;Benno, Y
通讯作者：
Benno, Y