Proto-oncogenic isoform A2 of eukaryotic translation elongation factor eEF1 is a target of miR-663 and miR-744.
Proto-oncogenic isoform A2 of eukaryotic translation elongation factor eEF1 is a target of miR-663 and miR-744.
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DOI：
10.1038/bjc.2013.243
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发表时间：
2013-06-11
影响因子：
8.8
通讯作者：
Groisman I
中科院分区：
文献类型：
--
作者：
Vislovukh A;Kratassiouk G;Porto E;Gralievska N;Beldiman C;Pinna G;El'skaya A;Harel-Bellan A;Negrutskii B;Groisman I
真核翻译延伸因子1A2 （eEF1A2）是一种已知的原癌基因。我们提出肿瘤组织中eEF1A2表达的刺激是由mirna介导的控制缺失引起的。分别通过qPCR和western blot检测miRNAs对eEF1A2 mRNA和蛋白水平的影响。采用双荧光素酶法检测mirna对EEF1A2 3′-UTR的影响。为了检测mirna结合位点，通过重叠延伸PCR将突变引入EEF1A2 mRNA的3 ' -UTR。miR-663和miR-744对附着在EEF1A2 3 ' -UTR上的荧光素酶基因的表达抑制作用分别高达20%和50%。在MCF7细胞中，过表达miR-663和miR-744可使EEF1A2 mRNA水平分别降低30%和50%。在eEF1A2蛋白水平上也观察到类似的效应。在白藜芦醇处理的MCF7细胞中，mir-663和mir-744的上调伴随着EEF1A2 mRNA的下调。这两种mirna都能抑制MCF7细胞的增殖。miR-663和miR-744介导原癌基因eEF1A2表达的抑制，导致MCF7癌细胞增殖迟缓。白藜芦醇的抗肿瘤作用可能包括刺激miR-663和miR-744的表达。
Eukaryotic translation elongation factor 1A2 (eEF1A2) is a known proto-oncogene. We proposed that stimulation of the eEF1A2 expression in cancer tissues is caused by the loss of miRNA-mediated control. Impact of miRNAs on eEF1A2 at the mRNA and protein levels was examined by qPCR and western blot, respectively. Dual-luciferase assay was applied to examine the influence of miRNAs on 3′-UTR of EEF1A2. To detect miRNA-binding sites, mutations into the 3′-UTR of EEF1A2 mRNA were introduced by the overlap extension PCR. miR-663 and miR-744 inhibited the expression of luciferase gene attached to the 3′-UTR of EEF1A2 up to 20% and 50%, respectively. In MCF7 cells, overexpression of miR-663 and miR-744 reduced the EEF1A2 mRNA level by 30% and 50%. Analogous effects were also observed at the eEF1A2 protein level. In resveratrol-treated MCF7 cells the upregulation of mir-663 and mir-744 was accompanied by downregulation of EEF1A2 mRNA. Both miRNAs were able to inhibit the proliferation of MCF7 cells. miR-663 and miR-744 mediate inhibition of the proto-oncogene eEF1A2 expression that results in retardation of the MCF7 cancer cells proliferation. Antitumour effect of resveratrol may include stimulation of the miR-663 and miR-744 expression.
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影响因子：
56.9
作者：
Lagos-Quintana, M;Rauhut, R;Tuschl, T
通讯作者：
Tuschl, T
DOI：
10.1111/j.1749-6632.2009.04909.x
发表时间：
2009-01-01
期刊：
NATURAL COMPOUNDS AND THEIR ROLE IN APOPTOTIC CELL SIGNALING PATHWAYS
影响因子：
--
作者：
Lee, Mee-Hyun;Surh, Young-Joon
通讯作者：
Surh, Young-Joon
影响因子：
14.9
作者：
Huang V;Place RF;Portnoy V;Wang J;Qi Z;Jia Z;Yu A;Shuman M;Yu J;Li LC
通讯作者：
Li LC
影响因子：
7.3
作者：
Lehmann, U.;Hasemeier, B.;Kreipe, H.
通讯作者：
Kreipe, H.
视黄酸通过上调 miR-663 诱导 HL-60 细胞分化
影响因子：
28.5
作者：
Jian, Pan;Li, Zhao Wen;Jian, Ni
通讯作者：
Jian, Ni