Effects of amiloride on gustatory neural responses to salts in the frog.
Effects of amiloride on gustatory neural responses to salts in the frog.
复制标题
阿米洛利对青蛙味觉神经对盐的反应的影响。
DOI：
10.1093/chemse/26.9.1203
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发表时间：
2001
期刊：
影响因子：
3.5
通讯作者：
Yoshihiro Mitoh
中科院分区：
文献类型：
--
作者：
Y. Kitada;K. Okuda;Yoshihiro Mitoh
在青蛙中，舌咽神经（GL）支配着几乎整个舌头上的味觉感受器。面神经的下颌支（MBF）和腭支（PN）分别支配舌底和上颚一小块区域的味觉感受器。本研究研究了上皮钠通道阻阻剂阿米洛利对蛙对NaCl、LiCl、KCl和CaCl的GL、MBF和PN的强直反应的影响(2)。在三种神经中，阿米洛利浓度为0.5 mM，相对较高，对0.15（略高于阈值）-0.5 M NaCl， 0.5 M LiCl和0.3 M KCl的反应没有影响，而对1.0 mM CaCl的反应几乎完全抑制(2)。由于对Ca（2+）的反应被其他阳离子(如Na（+）和Mg（2+）竞争性抑制，Amiloride可能通过Ca（2+）和Amiloride的一价阳离子之间的竞争性拮抗作用对CaCl(2)的反应发挥抑制作用。阿米洛利对GL、MBF和PN对NaCl的反应没有抑制作用，说明蛙类味觉受体细胞顶膜中阿米洛利敏感的钠通道不参与钠味觉传导。
In frogs, the glossopharyngeal nerve (GL) innervates taste receptors on almost the entire tongue. The mandibular branch (MBF) and palatine branch (PN) of the facial nerve innervate taste receptors on a very small area at the base of the tongue and on the palate, respectively. In the present study, effects of amiloride, an epithelial sodium channel blocker, on the tonic responses of the GL, MBF and PN in frogs to NaCl, LiCl, KCl and CaCl(2) were investigated. In three nerves, amiloride at 0.5 mM, a relatively high concentration, did not affect the responses to 0.15 (concentration just above threshold)-0.5 M NaCl, 0.5 M LiCl and 0.3 M KCl, whereas it almost completely inhibited the response to 1.0 mM CaCl(2). Amiloride may exert an inhibitory action on the response to CaCl(2) by a competitive antagonism between Ca(2+) and a monovalent cation of amiloride, because the response to Ca(2+) is competitively inhibited by other cations such as Na(+) and Mg(2+). The lack of inhibitory effect of amiloride on the responses in the GL, MBF and PN to NaCl suggests that amiloride-sensitive sodium channels in the apical membrane of taste receptor cells are not involved in sodium taste transduction in frogs.
影响因子：
56.9
作者：
Cha-Min Tang;Fernando Presser;M. Morad
通讯作者：
Cha-Min Tang;Fernando Presser;M. Morad
岩大浅神经的味觉反应：两种大鼠品系表现出明显的钠盐和阿米洛利敏感性。
影响因子：
1.9
作者：
Sollars,SI;Hill,DL
通讯作者：
Hill,DL