Stiff coatings on compliant biofibers: the cuticle of Mytilus californianus byssal threads.
Stiff coatings on compliant biofibers: the cuticle of Mytilus californianus byssal threads.
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DOI：
10.1021/bi900018m
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发表时间：
2009-03-31
期刊：
影响因子：
2.9
通讯作者：
Waite, J. Herbert
中科院分区：
文献类型：
--
作者：
Holten-Andersen, Niels;Zhao, Hua;Waite, J. Herbert
为了持久的附着，贻贝Mytilus californianus用2-5 μm厚的保护性角质层覆盖其深海丝，其硬度是下面的胶原纤维的4-6倍。虽然角质层硬度（0.1 GPa）和刚度（2 GPa）与相关贻贝中观察到的相似，但更有效的微损伤分散使M。californianus bysyal threads在角质层破裂发生之前将应变维持到几乎120%。深海角质层上级损伤耐受性的潜在因素在其微结构和角质层蛋白mcfp-1中进行了探索。角质层显微结构呈明显的颗粒状，颗粒直径（~ 200 nm）仅为M的1/4.比如说，galloprovincialis角质层。与近缘种贻贝中的同源蛋白相比，M. californianus具有相似的串联重复十肽的质量（约92 kDa）和数量，并且含有相同的翻译后修饰，即反式-4-羟基脯氨酸、反式-2，3-顺式-3，4-二羟基脯氨酸和3，4-二羟基苯丙氨酸（多巴）。然而，mcfp-1中异亮氨酸的突出性将其与其他物种中的同系物区分开来。从两个相关变体的cDNA推导的完整蛋白质序列揭示了高度保守的共有十肽PKISYPPTYK，其重复64次，并且与两个M的共有肽（AKPSYPPTYK）略有不同。galloprovincialis和M.美味蛋白
For lasting holdfast attachment, the mussel Mytilus californianus coats its byssal threads with a protective cuticle 2-5 μm thick that is 4-6 times stiffer than the underlying collagen fibers. Although cuticle hardness (0.1 GPa) and stiffness (2 GPa) resemble those observed in related mussels, a more effective dispersion of microdamage enables M. californianus byssal threads to sustain strains to almost 120% before cuticle rupture occurs. Underlying factors for the superior damage tolerance of the byssal cuticle were explored in its microarchitecture and in the cuticular protein, mcfp-1. Cuticle microstructure was distinctly granular, with granule diameters (∼200 nm) only a quarter of those in M. galloprovincialis cuticle, for example. Compared with homologous proteins in related mussel species, mcfp-1 from M. californianus had a similar mass (∼92 kDa) and number of tandemly repeated decapeptides, and contained the same post-translational modifications, namely, trans-4-hydroxyproline, trans-2,3-cis-3,4-dihydroxyproline, and 3,4-dihydroxyphenylalanine (Dopa). The prominence of isoleucine in mcfp-1, however, distinguished it from homologues in other species. The complete protein sequence deduced from cDNAs for two related variants revealed a highly conserved consensus decapeptide PKISYPPTYK that is repeated 64 times and differs slightly from the consensus peptide (AKPSYPPTYK) of both M. galloprovincialis and M. edulis proteins.
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影响因子：
4.8
作者：
Sun, CJ;Waite, JH
通讯作者：
Waite, JH
影响因子：
56.9
作者：
Miserez, Ali;Schneberk, Todd;Waite, J. Herbert
通讯作者：
Waite, J. Herbert
影响因子：
1.6
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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贻贝设计的防护涂层用于合规的底物。
影响因子：
7.6
作者：
Holten-Andersen, N.;Waite, J. H.
通讯作者：
Waite, J. H.