Bimodal Imaging of Mouse Peripheral Nerves with Chlorin Tracers.
Bimodal Imaging of Mouse Peripheral Nerves with Chlorin Tracers.
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用二氢卟酚示踪剂对小鼠周围神经进行双峰成像。
DOI：
10.1021/acs.molpharmaceut.0c00946
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发表时间：
2021-03-01
影响因子：
4.9
通讯作者：
Reiner T
中科院分区：
文献类型：
--
作者：
Gonzales J;Hernández-Gil J;Wilson TC;Adilbay D;Cornejo M;Demétrio de Souza Franca P;Guru N;Schroeder CI;King GF;Lewis JS;Reiner T
近1700万美国人有癌症史，预计到2030年将超过2200万。癌症患者经常接受化疗的形式，如顺铂和紫杉醇。虽然有效，但这些药物可能会引起使人衰弱的副作用;最常见的神经毒性作用，化疗诱导的周围神经病变（CIPN），可以在治疗结束后持续很长时间。尽管广泛和慢性的功能障碍的性质，没有工具存在定量测量化疗引起的周围神经病变。这样的工具不仅有利于患者，而且他们的分层也可以节省与神经性疼痛相关的重大财务和社会成本。在我们解决这一未满足的临床需求的第一步中，我们探索了一种新的双重方法来定位周围神经：切伦科夫发光成像（CLI）和荧光成像（FI）。我们的方法围绕电压门控钠通道亚型NaV1.7的靶向和成像，NaV1.7在收获的人类和小鼠组织的外周神经中高度表达。对于第一次，我们表明，热休克蛋白1a，放射性标记的NaV1.7-选择性肽分离自Homoeomma spec.秘鲁，可以作为一个有针对性的载体，提供一个放射性传感器到周围神经系统。在原位，我们观察到高信号噪声比的动物坐骨神经注射荧光标记的Hsp 1a和放射性标记的Hsp 1a。此外，对新鲜神经组织的共聚焦显微镜显示出同样高的荧光比率，证实了我们在体内的结果。这项研究表明，荧光标记和放射性标记的Hsp 1a示踪剂可用于在临床环境中识别和划分神经。
Almost 17 million Americans have a history of cancer, a number expected to reach over 22 million by 2030. Cancer patients often undergo chemotherapy in the form of antineoplastic agents such as cis-platin and paclitaxel. Though effective, these agents can induce debilitating side effects; the most common neurotoxic effect, chemotherapy-induced peripheral neuropathy (CIPN), can endure long after treatment ends. Despite the widespread and chronic nature of the dysfunction, no tools exist to quantitatively measure chemotherapy-induced peripheral neuropathy. Such a tool would not only benefit patients but their stratification could also save significant financial and social costs associated with neuropathic pain. In our first step toward addressing this unmet clinical need, we explored a novel dual approach to localize peripheral nerves: Cerenkov luminescence imaging (CLI) and fluorescence imaging (FI). Our approach revolves around the targeting and imaging of voltage-gated sodium channel subtype NaV1.7, highly expressed in peripheral nerves from both harvested human and mouse tissues. For the first time, we show that Hsp1a, a radiolabeled NaV1.7-selective peptide isolated from Homoeomma spec. Peru, can serve as a targeted vector for delivering a radioactive sensor to the peripheral nervous system. In situ, we observe high signal-to-noise ratios in the sciatic nerves of animals injected with fluorescently labeled Hsp1a and radiolabeled Hsp1a. Moreover, confocal microscopy on fresh nerve tissue shows the same high ratios of fluorescence, corroborating our in vivo results. This study indicates that fluorescently labeled and radiolabeled Hsp1a tracers could be used to identify and demarcate nerves in a clinical setting.
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影响因子：
3.8
作者：
Gebremedhn EG;Shortland PJ;Mahns DA
通讯作者：
Mahns DA
影响因子：
--
作者：
Das, Sudeep;Grimm, Jan;Thorek, Daniel L. J.
通讯作者：
Thorek, Daniel L. J.
影响因子：
15
作者：
Hoehne A;Behera D;Parsons WH;James ML;Shen B;Borgohain P;Bodapati D;Prabhakar A;Gambhir SS;Yeomans DC;Biswal S;Chin FT;Du Bois J
通讯作者：
Du Bois J
DOI：
10.1073/pnas.1109859108
发表时间：
2011-08-02
影响因子：
11.1
作者：
Reiner, Thomas;Thurber, Greg;Weissleder, Ralph
通讯作者：
Weissleder, Ralph
影响因子：
4.4
作者：
Nam, Jung Woo;Lee, Mi Jee;Kim, Hyung Jun
通讯作者：
Kim, Hyung Jun