Contrast-Enhanced Computed Tomography Enables Quantitative Evaluation of Tissue Properties at Intrajoint Regions in Cadaveric Knee Cartilage.
Contrast-Enhanced Computed Tomography Enables Quantitative Evaluation of Tissue Properties at Intrajoint Regions in Cadaveric Knee Cartilage.
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DOI：
10.1177/1947603516665443
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发表时间：
2017-10
期刊：
影响因子：
2.8
通讯作者：
Töyräs J
中科院分区：
文献类型：
--
作者：
Stewart RC;Honkanen JTJ;Kokkonen HT;Tiitu V;Saarakkala S;Joukainen A;Snyder BD;Jurvelin JS;Grinstaff MW;Töyräs J
本研究的目的是研究使用临床锥形束CT（CBCT）仪器通过对比增强计算机断层扫描（CECT）定量的阴离子造影剂碘克沙酸的浓度是否反映了在离体完整人体膝关节中成像的关节软骨的生物化学、组织学和生物力学特征。骨关节炎人尸体膝关节（91岁）注射碘克沙酸（36 mg I/mL），并在碘克沙酸扩散到软骨中的61小时内使用CBCT成像。成像后，切除关节表面，冲洗以去除造影剂，并通过压痕测试（n = 17个部位）测量压缩刚度（平衡和瞬时压缩模量）。对每个部位进行组织学切片，并使用番红-O染色切片的数字光密度测定法评估糖胺聚糖含量，使用Mankin和OARSI半定量评分系统评估胶原含量和形态学。使用冻干后的质量变化测定含水量。在所有成像时间点，包括碘克沙酸暴露<1小时的时间点，CECT衰减与软骨水和糖胺聚糖含量、Mankin评分以及平衡和瞬时压缩模量显著相关（P < 0.05）。早期时间点（<30分钟）与胶原含量和OARSI评分相关（P < 0.05）。在扩散平衡和短暂的碘克沙酸盐暴露后，关节内区域之间的软骨质量差异是可以区分的。使用碘克沙酸的CECT提供了软骨健康和性能的生化和生物力学测量，即使在短的临床相关暴露时间后，也可以在临床上用作检测软骨病理学早期体征的手段。
The aim of this study was to investigate whether the concentration of the anionic contrast agent ioxaglate, as quantitated by contrast-enhanced computed tomography (CECT) using a clinical cone-beam CT (CBCT) instrument, reflects biochemical, histological, and biomechanical characteristics of articular cartilage imaged in an ex vivo, intact human knee joint. An osteoarthritic human cadaveric knee joint (91 years old) was injected with ioxaglate (36 mg I/mL) and imaged using CBCT over 61 hours of ioxaglate diffusion into cartilage. Following imaging, the joint surfaces were excised, rinsed to remove contrast agent, and compressive stiffness (equilibrium and instantaneous compressive moduli) was measured via indentation testing (n = 17 sites). Each site was sectioned for histology and assessed for glycosaminoglycan content using digital densitometry of Safranin-O stained sections, Fourier transform infrared spectroscopy for collagen content, and morphology using both the Mankin and OARSI semiquantitative scoring systems. Water content was determined using mass change after lyophilization. CECT attenuation at all imaging time points, including those <1 hour of ioxaglate exposure, correlated significantly (P < 0.05) with cartilage water and glycosaminoglycan contents, Mankin score, and both equilibrium and instantaneous compressive moduli. Early time points (<30 minutes) also correlated (P < 0.05) with collagen content and OARSI score. Differences in cartilage quality between intrajoint regions were distinguishable at diffusion equilibrium and after brief ioxaglate exposure. CECT with ioxaglate affords biochemical and biomechanical measurements of cartilage health and performance even after short, clinically relevant exposure times, and may be useful in the clinic as a means for detecting early signs of cartilage pathology.
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影响因子：
2.4
作者：
Korhonen, RK;Laasanen, MS;Jurvelin, JS
通讯作者：
Jurvelin, JS
影响因子：
2.8
作者：
Kokkonen HT;Aula AS;Kröger H;Suomalainen JS;Lammentausta E;Mervaala E;Jurvelin JS;Töyräs J
通讯作者：
Töyräs J
影响因子：
62.1
作者：
Lusic, Hrvoie;Grinstaff, Mark W.
通讯作者：
Grinstaff, Mark W.
DOI：
10.1302/0301-620x.58b1.131804
发表时间：
1976-01-01
影响因子：
--
作者：
MCDEVITT, CA;MUIR, H
通讯作者：
MUIR, H
影响因子：
7
作者：
Lakin, B. A.;Grasso, D. J.;Snyder, B. D.
通讯作者：
Snyder, B. D.