Guilingji Protects Against Spermatogenesis Dysfunction From Oxidative Stress via Regulation of MAPK and Apoptotic Signaling Pathways in Immp2l Mutant Mice.
Guilingji Protects Against Spermatogenesis Dysfunction From Oxidative Stress via Regulation of MAPK and Apoptotic Signaling Pathways in Immp2l Mutant Mice.
复制标题
龟灵鸡通过调节 Immp2l 突变小鼠的 MAPK 和凋亡信号通路来预防氧化应激引起的生精功能障碍
DOI：
10.3389/fphar.2021.771161
复制
发表时间：
2021
影响因子：
5.6
通讯作者：
Liu G
中科院分区：
文献类型：
--
作者：
Wang Z;Xie Y;Chen H;Yao J;Lv L;Li Y;Deng C;Zhang M;Sun X;Liu G
男性不育症是一个主要的健康问题，估计全球男性不育症的患病率为4.2%。氧化应激（Oxidative stress，OS）是男性不育的主要原因之一，其特征是活性氧（reactive oxygen species，ROS）过多或抗氧化剂缺乏。同时，有报道称氧化应激在线粒体内膜肽酶2样（Immp 2l）突变小鼠的生精障碍中起重要作用。本研究旨在探讨龟龄集对Immp 2l突变小鼠生精功能的保护作用及其可能机制。结果显示，Immp 2l突变小鼠表现出精子发生受损，组织学显示生精小管中的生精细胞减少。龟龄集灌胃给药后，可减轻生精障碍，逆转睾丸组织病理学损伤，减少细胞凋亡。Western blotting和氧化还原经典标志物水平显示龟龄集能显著降低活性氧自由基。此外，龟龄集处理导致有丝分裂原活化蛋白激酶（MAPK）的磷酸化的抑制，调节细胞的凋亡。综上所述，龟龄集可通过调节氧化-抗氧化平衡和MAPK途径改善Immp 2l突变小鼠精子发生。我们的数据表明，龟龄集可能是一个有前途的和有效的抗氧化剂候选人治疗男性不育症。
Male infertility is a major health issue with an estimated prevalence of 4.2% of male infertility worldwide. Oxidative stress (OS) is one of the main causes of male infertility, which is characterized by excessive reactive oxygen species (ROS) or lack of antioxidants. Meanwhile, it is reported that oxidative stress plays an important role in the spermatogenic impairment in Inner mitochondrial membrane peptidase 2-like (Immp2l) mutant mice. In this study, we focused on the potential mechanism of Guilingji in protecting the spermatogenic functions in Immp2l mutant mice. The results revealed that Immp2l mutant mice exhibit impaired spermatogenesis and histology shows seminiferous tubules with reduced spermatogenic cells. After administration of Guilingji [150 mg/kg per day intragastric gavage], however, alleviated spermatogenesis impairment and reversed testis histopathological damage and reduced apoptosis. What’s more, western blotting and the levels of redox classic markers revealed that Guilingji can markedly reduce reactive oxygen species. Moreover, Guilingji treatment led to inhibition of the phosphorylation of mitogen-activated protein kinase (MAPK), regulated apoptosis in the cells. In summary, Guilingji can improve spermatogenesis in Immp2l mutant mice by regulating oxidation-antioxidant balance and MAPK pathway. Our data suggests that Guilingji may be a promising and effective antioxidant candidate for the treatment of male infertility.
登录
查看更多内容
影响因子：
13.6
作者：
Li MW;Mruk DD;Cheng CY
通讯作者：
Cheng CY
影响因子：
8.2
作者：
Shi, Jing;Xue, Wei;Li, Ke-xin
通讯作者：
Li, Ke-xin
影响因子：
168.9
作者：
Holden, CA;McLachlan, RI;de Kretser, DM
通讯作者：
de Kretser, DM
DOI：
10.5534/wjmh.2014.32.1.1
发表时间：
2014-04
期刊：
The world journal of men's health
影响因子：
--
作者：
Agarwal A;Virk G;Ong C;du Plessis SS
通讯作者：
du Plessis SS
影响因子：
--
作者：
Greco, E;Iacobelli, M;Tesarik, J
通讯作者：
Tesarik, J