Altered antioxidant status and increased lipid per-oxidation in seminal plasma of tunisian infertile men.
Altered antioxidant status and increased lipid per-oxidation in seminal plasma of tunisian infertile men.
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DOI：
10.7150/ijbs.8.139
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发表时间：
2012
影响因子：
9.2
通讯作者：
Ajina M
中科院分区：
文献类型：
--
作者：
Atig F;Raffa M;Ali HB;Abdelhamid K;Saad A;Ajina M
人类精浆是天然的抗氧化剂储存库，可保护精子免受氧化损伤。文献证据支持精液抗氧化和脂质过氧化状态的损害在男性不育的生理病理中起重要作用。我们目前的研究是在突尼斯进行的第一项研究。我们首次对120名接受体外受精（IVF）的不育男性的精浆中抗氧化状态进行了评估。患者以特发性不孕症为特征。他们被分为三组：正常精子者作为对照组（n=40），弱精子者（Astheno, n=45）和低弱异精子者（OAT, n=35）。测定种子超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPX）活性以及谷胱甘肽（GSH）、锌（Zn）和丙二醛（MDA）水平。正常精子组SOD和GPX活性显著升高，与精子质量呈正相关。在对照组中，精液锌和谷胱甘肽的含量显著升高，但这些抗氧化剂对精液参数的影响存在矛盾的关联。然而，我们注意到在精原图异常组，丙二醛水平显著升高。我们发现这种氧化标记物与精子参数之间存在负相关。这些结果明显表明，精子抗氧化剂的损伤是与特发性不孕症相关的精子质量低下的重要危险因素，因此可能导致试管受精结果不佳。
Human seminal plasma is a natural reservoir of antioxidants that protect spermatozoa from oxidative damages. There is evidence in literature supports the fact that impairments in seminal antioxidant and lipid per-oxidation status play important roles in the physiopathology of male infertility. Our present study forms the first one which was carried out in Tunisia. We evaluated the antioxidant status in the seminal plasma of 120 infertile men programmed to In Vitro Fertilization (IVF) for the first tentative. Patients were characterized by an idiopathic infertility. They were divided into three groups: normozoospermics who were considered as controls (n=40), asthenozoospermics (Astheno; n=45) and oligoasthenoteratozoospermics (OAT; n=35). Seminal activities of superoxide dismutase (SOD) and glutathione peroxidase (GPX) and the levels of glutathione (GSH), zinc (Zn) and malondialdehyde (MDA) were measured. With the significant increase of the seminal activities of SOD and GPX in normozoospermics group, there were positive correlations observed between this enzymes and sperm quality. Also, significant elevated rates of seminal zinc and GSH were observed in control group, but there was contradictory associations reflecting the effects of these antioxidants on semen parameters. However, we noted significant increase of MDA levels in groups with abnormal seminogram. We showed negative associations between this per-oxidative marker and sperm parameters. These results obviously suggested that impairment on seminal antioxidants is an important risk factor for low sperm quality associated to idiopathic infertility and as a result can lead to poor IVF outcome.
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DOI：
10.1016/s0306-3623(97)00072-4
发表时间：
1998-01-01
期刊：
GENERAL PHARMACOLOGY
影响因子：
--
作者：
Bagchi, D;Vuchetich, PJ;Stohs, SJ
通讯作者：
Stohs, SJ
影响因子：
4.5
作者：
Colagar, Abasalt Hosseinzadeh;Marzony, Eisa Tahmasbpour;Chalchi, Mohammad Javad
通讯作者：
Chalchi, Mohammad Javad
DOI：
10.1002/mrd.1120230108
发表时间：
1989-05-01
期刊：
GAMETE RESEARCH
影响因子：
--
作者：
ALVAREZ, JG;STOREY, BT
通讯作者：
STOREY, BT
影响因子：
2.1
作者：
CALDAMONE, AA;FREYTAG, MK;COCKETT, ATK
通讯作者：
COCKETT, ATK
影响因子：
4.1
作者：
Hall, L;Williams, K;Jury, JA
通讯作者：
Jury, JA