Influenza A Virus Inhibits RSV Infection via a Two-Wave Expression of IFIT Proteins.
Influenza A Virus Inhibits RSV Infection via a Two-Wave Expression of IFIT Proteins.
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流感病毒通过IFIT蛋白的两波表达抑制RSV感染。
DOI：
10.3390/v12101171
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发表时间：
2020-10-16
期刊：
影响因子：
--
通讯作者：
Mandelboim M
中科院分区：
文献类型：
--
作者：
Drori Y;Jacob-Hirsch J;Pando R;Glatman-Freedman A;Friedman N;Mendelson E;Mandelboim M
流感病毒和呼吸道合胞病毒（RSV）是呼吸道病毒，主要在秋冬季节在全球传播。季节性监测表明，呼吸道合胞病毒感染通常先于流感。然而，在过去的四个冬季（2016-2020年），以色列观察到两种病毒的发病率高峰重叠，与此同时，呼吸道合胞病毒感染率显著降低。为了研究甲型流感病毒是否能抑制RSV，我们将人宫颈癌（HEp2）细胞或小鼠同时感染甲型流感病毒和RSV。甲型流感在体内和体外均抑制RSV的生长。对感染甲型流感的小鼠肺部进行质谱分析，发现了一种蛋白表达上调的双波模式，其中包括干扰素诱导蛋白的四肽（IFITs）家族成员。有趣的是，在第二波中，在小鼠肺部不再检测到甲型流感病毒。此外，HEp2细胞中IFITs的敲低和过表达会影响RSV的多样性。综上所述，甲型流感感染通过双波模式上调IFIT蛋白抑制RSV传染性。了解免疫系统参与甲型流感和RSV病毒之间的相互作用将有助于制定未来针对这些病毒的治疗策略。
Influenza viruses and respiratory syncytial virus (RSV) are respiratory viruses that primarily circulate worldwide during the autumn and winter seasons. Seasonal surveillance has shown that RSV infection generally precedes influenza. However, in the last four winter seasons (2016–2020) an overlap of the morbidity peaks of both viruses was observed in Israel, and was paralleled by significantly lower RSV infection rates. To investigate whether the influenza A virus inhibits RSV, human cervical carcinoma (HEp2) cells or mice were co-infected with influenza A and RSV. Influenza A inhibited RSV growth, both in vitro and in vivo. Mass spectrometry analysis of mouse lungs infected with influenza A identified a two-wave pattern of protein expression upregulation, which included members of the interferon-induced protein with the tetratricopeptide (IFITs) family. Interestingly, in the second wave, influenza A viruses were no longer detectable in mouse lungs. In addition, knockdown and overexpression of IFITs in HEp2 cells affected RSV multiplicity. In conclusion, influenza A infection inhibits RSV infectivity via upregulation of IFIT proteins in a two-wave modality. Understanding the immune system involvement in the interaction between influenza A and RSV viruses will contribute to the development of future treatment strategies against these viruses.
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影响因子：
8
作者：
通讯作者：
--
影响因子：
4.6
作者：
Chen X;Xu B;Guo J;Li C;An S;Zhou Y;Chen A;Deng L;Fu Z;Zhu Y;Liu C;Xu L;Wang W;Shen K;Xie Z
通讯作者：
Xie Z
DOI：
10.1111/j.1432-1033.1994.00877.x
发表时间：
1994-09-15
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
KITAMURA, A;TAKAHASHI, K;KITAMURA, N
通讯作者：
KITAMURA, N
影响因子：
4.4
作者：
Grayson, Mitchell H.;Ramos, Madeleine S.;Holtzman, Michael J.
通讯作者：
Holtzman, Michael J.
影响因子：
14.9
作者：
Kumar P;Sweeney TR;Skabkin MA;Skabkina OV;Hellen CU;Pestova TV
通讯作者：
Pestova TV