Extensive recombination events and horizontal gene transfer shaped the Legionella pneumophila genomes.
Extensive recombination events and horizontal gene transfer shaped the Legionella pneumophila genomes.
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DOI：
10.1186/1471-2164-12-536
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发表时间：
2011-11-01
期刊：
影响因子：
4.4
通讯作者：
Buchrieser C
中科院分区：
文献类型：
--
作者：
Gomez-Valero L;Rusniok C;Jarraud S;Vacherie B;Rouy Z;Barbe V;Medigue C;Etienne J;Buchrieser C
嗜肺军团菌是环境原生动物的胞内病原体。当人类吸入被污染的气溶胶时，这种细菌可能会引起严重的肺炎，称为军团病。尽管在水生水库中有大量的军团菌，但绝大多数人类疾病是由单一物种的单一血清群（Sg）引起的，即L。嗜肺菌Sg 1.为了进一步了解Sg 1菌株的基因组动态和进化，我们对菌株Lorraine和HL 0604 1035（Sg 1）进行了测序，并将其与Sg 1菌株巴黎、透镜、Corby和Philadelphia的可用序列进行了比较，从而进行了全面的多基因组分析。我们证明了L.嗜肺菌Sg 1具有高度保守和同线的核心基因组，其包含许多真核样蛋白和超过200个Dot/Icm IV型分泌底物的保守库。然而，重组事件和水平基因转移是频繁的。特别是对核苷酸多态性分布的分析表明，在L. pneumophila菌株，并有助于在自然种群的基因组动态。存在的许多分泌系统可能涉及通过接合转移交换这些片段。质粒酶还在基因组多样化中发挥作用，并在菌株之间交换，并在不同的军团菌属物种之间循环。细菌间的水平基因转移以及真核生物向乳酸杆菌的水平基因转移。嗜肺菌之间的相互作用以及菌株之间的重组允许不同的克隆进化成主要的疾病克隆，并且其他克隆随后在相对短的时间内取代它们。
Legionella pneumophila is an intracellular pathogen of environmental protozoa. When humans inhale contaminated aerosols this bacterium may cause a severe pneumonia called Legionnaires' disease. Despite the abundance of dozens of Legionella species in aquatic reservoirs, the vast majority of human disease is caused by a single serogroup (Sg) of a single species, namely L. pneumophila Sg1. To get further insights into genome dynamics and evolution of Sg1 strains, we sequenced strains Lorraine and HL 0604 1035 (Sg1) and compared them to the available sequences of Sg1 strains Paris, Lens, Corby and Philadelphia, resulting in a comprehensive multigenome analysis. We show that L. pneumophila Sg1 has a highly conserved and syntenic core genome that comprises the many eukaryotic like proteins and a conserved repertoire of over 200 Dot/Icm type IV secreted substrates. However, recombination events and horizontal gene transfer are frequent. In particular the analyses of the distribution of nucleotide polymorphisms suggests that large chromosomal fragments of over 200 kbs are exchanged between L. pneumophila strains and contribute to the genome dynamics in the natural population. The many secretion systems present might be implicated in exchange of these fragments by conjugal transfer. Plasmids also play a role in genome diversification and are exchanged among strains and circulate between different Legionella species. Horizontal gene transfer among bacteria and from eukaryotes to L. pneumophila as well as recombination between strains allows different clones to evolve into predominant disease clones and others to replace them subsequently within relatively short periods of time.
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影响因子：
2.8
作者：
Edwards, Martin T.;Fry, Norman K.;Harrison, Timothy G.
通讯作者：
Harrison, Timothy G.
影响因子：
2.8
作者：
Doleans-Jordheim, Anne;Akermi, Mongi;Jarraud, Sophie
通讯作者：
Jarraud, Sophie
影响因子：
2.6
作者：
Bumbaugh, AC;McGraw, EA;Whittam, TS
通讯作者：
Whittam, TS
影响因子：
56.9
作者：
Chien, MC;Morozova, I;Russo, JJ
通讯作者：
Russo, JJ
DOI：
10.1099/ijs.0.63572-0
发表时间：
2005-09-01
影响因子：
2.8
作者：
Collingro, A;Toenshoff, ER;Horn, M
通讯作者：
Horn, M