Characterization of a dorsal-eye Gal4 Line in Drosophila.
Characterization of a dorsal-eye Gal4 Line in Drosophila.
复制标题
DOI:
10.1002/dvg.20571
复制
发表时间:
2010-01
期刊:
影响因子:
1.5
通讯作者:
Halder, Georg
中科院分区:
文献类型:
--
作者:
Morrison, Clayton M.;Halder, Georg
In Drosophila, the Gal4-UAS system is used to drive ectopic gene expression in a tissue-specific manner. In this system, transgenic flies expressing tissue specific Gal4 are crossed to a line in which the gene to be expressed is under the control of a Gal4-responsive UAS sequence. The resulting progeny express the gene of interest in the pattern of the particular Gal4 line. Since a given UAS-transgene can be driven by any Gal4 line, this system is predominantly limited by available Gal4 lines. Here we report the characterization of a novel line, DE-Gal4, which in the eye is expressed in the dorsal compartment for the majority of development. Furthermore, we use functional tests to show that the DE-Gal4 line is a useful tool with which to manipulate gene expression in half of the developing eye.
登录
查看更多内容
影响因子:
64.5
作者:
HIROMI, Y;KUROIWA, A;GEHRING, WJ
通讯作者:
GEHRING, WJ
影响因子:
2.7
作者:
Croker, JA;Ziegenhorn, SL;Holmgren, RA
通讯作者:
Holmgren, RA
影响因子:
64.5
作者:
Prober, DA;Edgar, BA
通讯作者:
Edgar, BA
影响因子:
11.8
作者:
Zhang, Lei;Ren, Fangfang;Jiang, Jin
通讯作者:
Jiang, Jin
影响因子:
11.4
作者:
HARRISON, DA;BINARI, R;PERRIMON, N
通讯作者:
PERRIMON, N