Characterization of a dorsal-eye Gal4 Line in Drosophila.

Characterization of a dorsal-eye Gal4 Line in Drosophila.
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DOI:
10.1002/dvg.20571
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发表时间:
2010-01
期刊:
影响因子:
1.5
通讯作者:
Halder, Georg
Halder, Georg
中科院分区:
生物学4区
文献类型:
--
作者:
Morrison, Clayton M.;Halder, Georg

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在果蝇中,Gal 4-UAS系统用于以组织特异性方式驱动异位基因表达。在该系统中,将表达组织特异性Gal 4的转基因果蝇与其中待表达的基因在Gal 4响应性UAS序列的控制下的品系杂交。所得后代以特定Gal 4系的模式表达目的基因。由于给定的UAS转基因可以由任何Gal 4系驱动,因此该系统主要受到可用Gal 4系的限制。在这里,我们报告了一个新的线,DE-Gal 4,这在眼睛中是在背侧室的大部分发展的表征。此外,我们使用功能测试表明,DE-Gal 4线是一个有用的工具,其中操纵基因表达的一半发展中的眼睛。
In Drosophila, the Gal4-UAS system is used to drive ectopic gene expression in a tissue-specific manner. In this system, transgenic flies expressing tissue specific Gal4 are crossed to a line in which the gene to be expressed is under the control of a Gal4-responsive UAS sequence. The resulting progeny express the gene of interest in the pattern of the particular Gal4 line. Since a given UAS-transgene can be driven by any Gal4 line, this system is predominantly limited by available Gal4 lines. Here we report the characterization of a novel line, DE-Gal4, which in the eye is expressed in the dorsal compartment for the majority of development. Furthermore, we use functional tests to show that the DE-Gal4 line is a useful tool with which to manipulate gene expression in half of the developing eye.
DOI: 10.1016/0092-8674(85)90232-6
发表时间: 1985-12-01
期刊: CELL
影响因子: 64.5
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