The dominant Drop eye mutations of Drosophila melanogaster define two loci implicated in normal eye development
The dominant Drop eye mutations of Drosophila melanogaster define two loci implicated in normal eye development
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果蝇的显性眼下垂突变定义了与正常眼睛发育有关的两个基因座
DOI：
10.1007/bf00286695
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
R. Saint
中科院分区：
文献类型：
--
作者：
R. Tearle;A. Tomlinson;R. Saint
现有的三个显性功能获得性Drop等位基因Dr 1、DrMio和DrWe，以前被认为定义了一个单一的基因座，严重破坏了眼睛的发育。甲基磺酸乙酯（EMS）和辐射诱导的回复突变体的遗传分析表明，下降突变定义两个位点：下降位点，这是由Dr 1和DrMio突变体定义，和一个单独的位点定义的DrWe突变，已更名为楔。大多数Dr 1和DrMio回复突变体是胚胎致死的反式突变胚胎，表现出气管未能加入Filzkörper，从而揭示了Drop基因在胚胎发生中的作用。致死回复突变等位基因的克隆分析表明，这两个基因在眼睛发育中的作用。在Drop纯合突变体克隆中，外感光细胞R1-R6发育异常。然而，楔并不是发育中的感光细胞所必需的，但它的缺失确实会破坏正常的小眼排列。虽然Drop和附近的弦位点被证明是遗传上不同的，但Dr 1和DrMio都被发现与弦位点的病变相互作用，导致刚毛丢失和紊乱以及神经肌肉协调的丧失。
The three existing dominant gain-of-function Drop alleles, Dr1, DrMioand DrWe, previously assumed to define a single locus, severely disrupt eye development. Genetic analysis of ethylmethanesulphonate (EMS) and irradiation-induced revertants revealed that the Drop mutations define two loci: the Drop locus, which is defined by the Dr1 and DrMio mutants, and a separate locus defined by the DrWe mutation, which has been renamed Wedge. The majority of the Dr1 and DrMio revertants are embryonic lethal in trans, mutant embryos exhibiting trachea that fail to join the Filzkörper, thus revealing a role for the Drop gene in embryogenesis. Clonal analysis of lethal revertant alleles suggests a role for both genes in eye development. In the Drop homozygous mutant clones, the outer photoreceptor cells R1–R6 develop aberrantly. Wedge, however, is not required by the developing photoreceptor cells but its absence does disrupt normal ommatidial alignment. Although the Drop and nearby string loci were shown to be genetically distinct, both Dr1 and DrMio were found to interact in trans with lesions at the string locus, causing loss and derangement of bristles and loss of neuromuscular coordination.
影响因子：
10.5
作者：
CAGAN, RL;READY, DF
通讯作者：
READY, DF
影响因子：
2.7
作者：
CAGAN, RL;READY, DF
通讯作者：
READY, DF