Thermal sensitivity of endothelial cells on synthetic vascular graft material.
Thermal sensitivity of endothelial cells on synthetic vascular graft material.
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DOI：
10.3109/02656736.2011.638963
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发表时间：
2012
影响因子：
3.1
通讯作者：
Christensen, Douglas A.
中科院分区：
文献类型：
--
作者：
Brinton, Mark R.;Tagge, Chad A.;Stewart, Russell J.;Cheung, Alfred K.;Shiu, Yan-Ting E.;Christensen, Douglas A.
目的是确定热暴露能够减少或消除膨胀聚四氟乙烯（ePTFE）的细胞存活，以开发一种轻度热疗治疗ePTFE血管移植物中内膜增生的方法。在不同的热暴露条件下，对活的和死的牛主动脉内皮细胞进行量化：分别在42°和45°C下加热胶原包被ePTFE片或组织培养聚苯乙烯培养皿上的细胞，测定其在不同表面的热敏性，在43-50°C下加热不同时间，然后在37°C下孵育0和20 h。显著细胞死亡设置为50%。通过细胞形态和膜完整性评估区分凋亡和坏死两种类型的细胞死亡。细胞在ePTFE片上的附着和存活受轻度加热抑制的敏感性高于组织培养皿。暴露在45°C下90分钟和50°C下30分钟导致ePTFE上显著的坏死细胞死亡（分别为65%和75%）。在47°C和50°C下暴露30分钟后，37°C/20小时的孵育使总细胞死亡率（坏死+凋亡）分别从20%增加到50%和75%增加到100%。与组织培养皿上的细胞相比，在ePTFE上生长的细胞更容易受到轻度高温诱导的死亡。通过优化暴露温度和暴露时间，ePTFE上的细胞主要通过凋亡死亡。
The goal is to identify thermal exposures capable of reducing or eliminating cell survival on expanded polytetrafluoroethylene (ePTFE), in an effort to develop a mild hyperthermia treatment of neointimal hyperplasia in ePTFE vascular grafts. Viable and dead bovine aortic endothelial cells were quantified following different thermal exposure conditions: cells on collagen-coated ePTFE sheets or tissue culture polystyrene dishes were heated at 42°and 45°C to determine their thermal sensitivity on different surfaces, and cells cultured on collagen-coated ePTFE sheets were heated at 43–50°C for various durations, followed by incubation at 37°C for 0 and 20 h, respectively. Significant cell death was set to be 50%. Two types of cell death, apoptosis and necrosis, were distinguished by cell morphology and membrane integrity assessments. The attachment and survival of cells on ePTFE sheets were more sensitive to inhibition by mild heating than those on tissue culture dishes. Exposure to 45°C for 90 min and 50°C for 30 min caused significant necrotic cell death on ePTFE (65% and 75%, respectively). A 37°C/20-h incubation following 30-min exposures at 47°and 50°C increased total cell death (necrosis + apoptosis) from 20% to 50% and 75% to 100%, respectively. Cells grown on ePTFE were more susceptible to mild hyperthermia-induced death, compared to those on tissue culture dishes. Significant cell death on ePTFE mainly via apoptosis can be achieved by optimising temperature and duration of exposure.
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影响因子：
7.5
作者：
Chicurel, ME;Chen, CS;Ingber, DE
通讯作者：
Ingber, DE
影响因子：
37.8
作者：
Colombo, A;Drzewiecki, J;Russell, ME
通讯作者：
Russell, ME
影响因子：
7.5
作者：
Galbraith, CG;Sheetz, MP
通讯作者：
Sheetz, MP
影响因子：
37.8
作者：
HEHRLEIN, C;CHI, HC;SVENSON, RH
通讯作者：
SVENSON, RH
影响因子：
8.3
作者：
Champagne, MJ;Dumas, P;Tremblay, J
通讯作者：
Tremblay, J