Coating with flexible DNA network enhanced T-cell activation and tumor killing for adoptive cell therapy.
Coating with flexible DNA network enhanced T-cell activation and tumor killing for adoptive cell therapy.
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用柔性DNA网络涂层增强了T细胞活化和杀死肿瘤的涂层，以进行过养细胞疗法。
DOI：
10.1016/j.apsb.2021.04.002
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发表时间：
2021-07
期刊：
影响因子：
--
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Zhang Z;Liu Q;Tan J;Zhan X;Liu T;Wang Y;Lu G;Wu M;Zhang Y
Adoptive cell therapy (ACT) is an emerging powerful cancer immunotherapy, which includes a complex process of genetic modification, stimulation and expansion. During these in vitro or ex vivo manipulation, sensitive cells are inescapability subjected to harmful external stimuli. Although a variety of cytoprotection strategies have been developed, their application on ACT remains challenging. Herein, a DNA network is constructed on cell surface by rolling circle amplification (RCA), and T cell-targeted trivalent tetrahedral DNA nanostructure is used as a rigid scaffold to achieve high-efficient and selective coating for T cells. The cytoprotective DNA network on T-cell surface makes them aggregate over time to form cell clusters, which exhibit more resistance to external stimuli and enhanced activities in human peripheral blood mononuclear cells and liver cancer organoid killing model. Overall, this work provides a novel strategy for in vitro T cell-selective protection, which has a great potential for application in ACT. T cell-selective coating and protection strategy with a flexible DNA network was formed by rolling circle amplification, which promotes T-cell aggregation and activation for anti-tumor therapy.
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影响因子：
4.6
作者：
Jeong H;Hwang J;Lee H;Hammond PT;Choi J;Hong J
通讯作者：
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影响因子：
13.3
作者：
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影响因子：
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DOI：
10.1016/j.apsb.2021.02.009
发表时间：
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期刊：
Acta pharmaceutica Sinica. B
影响因子：
--
作者：
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通讯作者：
Qi X
影响因子：
6
作者：
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通讯作者：
Dive, Caroline