A unique transcriptome: 1782 positions of RNA editing alter 1406 codon identities in mitochondrial mRNAs of the lycophyte Isoetes engelmannii.
A unique transcriptome: 1782 positions of RNA editing alter 1406 codon identities in mitochondrial mRNAs of the lycophyte Isoetes engelmannii.
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DOI：
10.1093/nar/gkq1227
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发表时间：
2011-04
影响因子：
14.9
通讯作者：
Knoop V
中科院分区：
文献类型：
--
作者：
Grewe F;Herres S;Viehöver P;Polsakiewicz M;Weisshaar B;Knoop V
作为石松植物的第一个代表的Isopelengelmannii的线粒体DNA的分析最近揭示了非常小的内含子和非常频繁的RNA编辑的迹象。分析I. engelmannii，我们对其线粒体转录组进行了全面的分析。发现所有30个组I和II内含子被正确地去除，表明内含子大小的减小并不妨碍剪接。我们发现，mRNA编辑影响1782个位点，这导致密码子含义的1406个变化。这包括从25个线粒体蛋白质编码基因中的23个去除终止密码子。每个基因座的多个cDNA的综合序列分析允许将部分编辑的位点分类为低效编辑但相关的或以大多数低频率非特异性编辑的。丰富的RNA编辑也被发现以前所未有的频率影响tRNA，发生在tRNA前体的41个位置上，包括在两种tRNA物种中首次发现U到C交换。最后，我们研究了nad7基因的四个II组内含子，并确定了27个编辑位点，其中大部分改善了碱基配对，以形成适当的二级结构。
The analysis of the mitochondrial DNA of Isoetes engelmannii as a first representative of the lycophytes recently revealed very small introns and indications for extremely frequent RNA editing. To analyze functionality of intron splicing and the extent of RNA editing in I. engelmannii, we performed a comprehensive analysis of its mitochondrial transcriptome. All 30 groups I and II introns were found to be correctly removed, showing that intron size reduction does not impede splicing. We find that mRNA editing affects 1782 sites, which lead to a total of 1406 changes in codon meanings. This includes the removal of stop codons from 23 of the 25 mitochondrial protein encoding genes. Comprehensive sequence analysis of multiple cDNAs per locus allowed classification of partially edited sites as either inefficiently edited but relevant or as non-specifically edited at mostly low frequencies. Abundant RNA editing was also found to affect tRNAs in hitherto unseen frequency, taking place at 41 positions in tRNA-precursors, including the first identification of U-to-C exchanges in two tRNA species. We finally investigated the four group II introns of the nad7 gene and could identify 27 sites of editing, most of which improve base pairing for proper secondary structure formation.
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影响因子：
5.5
作者：
Jobson RW;Qiu YL
通讯作者：
Qiu YL
影响因子：
10.7
作者：
Chaw, Shu-Miaw;Shih, Arthur Chun-Chieh;Chou, The-Yuan
通讯作者：
Chou, The-Yuan
影响因子：
3.3
作者：
Bentolila, Stephane;Elliott, Leah E.;Hanson, Maureen R.
通讯作者：
Hanson, Maureen R.
影响因子：
10.7
作者：
Groth-Malonek, Milena;Wahrmund, Ute;Knoop, Volker
通讯作者：
Knoop, Volker
影响因子：
3.5
作者：
BLANC, V;LITVAK, S;ARAYA, A
通讯作者：
ARAYA, A