Hybridoma Technology in the Biosciences and Medicine-猫眼课题宝

生物科学和医学中的杂交瘤技术

DOI：

发表时间：

2012

期刊：

Springer US

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作者：

T. Springer

作者：Timothy a .施普林格，全会出版公司1985,75美元（xxv + 602页）ISBN 0 306 41996 3这是一本好书。Dr施普林格汇集了一系列杰出作者的文章，涵盖了单克隆抗体（mAb）的生产和使用的大多数方面。它主要不是一本实验方案的“食谱”，尽管有几章确实包含了详细的方法，但它是一系列解释文献并展示如何使用该技术的小型评论。主要有五个部分。第一部分“单克隆抗体的基因工程生产和改进”涵盖了小鼠和人虹膜瘤的生产。它还包含如何获得变异抗体的信息，如果它不是完全令人满意的首次生产。然而，对我来说，最有趣的章节是关于克隆免疫球蛋白基因的表达。第二部分涵盖了单克隆抗体在生物医学研究中的一些应用。包括细胞表面受体研究的许多方面，以及病毒上的抗原决定因子和细胞上的病毒受体的划定。Parham有一个很有价值的章节，涵盖了生产单克隆片段的技术，以及抗体与细胞表面的结合。第三部分概述了一些用于克隆和转染基因的技术。它在使用抗体纯化特定蛋白质的信使RNA和克隆后检测基因产物的表达方面尤其强大。第四部分包括四章，论述了在诊断和治疗中的应用。这是书中篇幅最有限的部分。它只涵盖了使用单克隆抗体使用补体固定抗体清除骨髓中不需要的细胞，用单克隆抗体治疗器官移植和白血病，并非常简短地概述了它们在去除有毒药物或可视化特定病理过程（如梗死或血栓）方面的潜在用途。任何寻求单克隆抗体临床应用的更充分的讨论应该咨询McMichael和Fabre的单克隆抗体在临床医学，这是最接近的竞争对手为这一卷。本书的最后一部分描述了t细胞的一些用途！杂交瘤和t细胞克隆作为生物活性产品的来源。概述了它们在定义t细胞抗原受体和细胞溶解过程的研究中的应用。总之，这本书提供了在快速发展的生物技术领域的若干主题的背景信息。因此，它将对许多生物科学的研究生和有兴趣了解抗体可以用来解决问题的方法的物理学家研究人员有价值。它不是，也不倾向于是详细的方法目录或临床应用的综合清单。我喜欢读这本书，从中我学到了很多东西。

by Timothy A. Springer, Plenum Publishing Corporation 1985, US$ 75.00 (xxv + 602 pages) ISBN 0 306 41996 3 This is a good book. Dr Springer has assembled a collection of articles by distinguished authors which covers most aspects of the production and use of monoclonal antibodies (mAb). it is not primarily a 'cookbook' of experimental protocols, although a few chapters do contain detailed methods, but a series of minireviews which explain the literature and show how the technology can be used. There are five main sections. Part I entitled 'The production and improvement through genetic en- gineering of monoclonal antibodies' covers both mouse and human hy- bridoma production. It also contains information on how to obtain variants of the antibody first pro- duced if it isn't entirely satisfactory. To me, however, the most interest- ing chapter was that by AIt on the expression of cloned immunoglobu- lin genes. Part II covers some of the many applications of monoclonal anti- bodies to biomedical research. Many aspects of cell surface receptor stud- ies are covered as well as the de- lineation of antigenic determinants on viruses and the receptors for viruses on cells. There is a valuable chapter by Parham covering tech- niques for producing fragments of monoclonals as well as the binding of antibodies to cell surfaces. Part III outlines some of the tech- niques used to clone and transfect genes. It is particularly strong on the use of antibodies either to purify messenger RNA for specific proteins and to detect expression of the gene product after cloning. Part IV contains four chapters on applications in diagnosis and ther- apy. This is the most limited section of the book. It covers only the use of mAb to clear bone marrow of un- wanted cells using complement fix- ing -antibodies, the therapy of organ grafts and leukemias with mAb, and a very short outline of their potential use to remove toxic drugs or to visualize specific pathological pro- cesses such as infarcts or thrombi. Anyone seeking a fuller discussion of the clinical uses of mAbs should consult McMichael and Fabre's Monoclonal Antibodies in Clinical Medicine which is the closest com- petitor for this volume. The final part of this book de- scribes some of the uses of T-cel! hybridomas and T-cell clones as sources of biologically active prod- ucts. An overview of their use in defining the T-cell antigen receptor and the study of the cytolytic process is included. In summary, this book provides background information on a num- ber of topics in rapidly evolving areas of biotechnology. As such it would be of value to many graduate stu- dents in the biosciences and phys- ician investigators interested in understanding the ways in which antibodies can be used to solve problems. It is not, and is not in- tended to be, a catalogue of detailed methodology or a comprehensive list of clinical applications. I enjoyed reading it and I learned several things from it.