Genome-wide analysis of circular RNAs in prenatal and postnatal muscle of sheep.
Genome-wide analysis of circular RNAs in prenatal and postnatal muscle of sheep.
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DOI：
10.18632/oncotarget.21835
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发表时间：
2017-11-14
期刊：
影响因子：
--
通讯作者：
Hu S
中科院分区：
文献类型：
--
作者：
Li C;Li X;Yao Y;Ma Q;Ni W;Zhang X;Cao Y;Hazi W;Wang D;Quan R;Hou X;Liu Z;Zhan Q;Liu L;Zhang M;Yu S;Hu S
环状RNA（Circular RNA，circRNA）是一类通过反向剪接产生的具有环状结构的非编码RNA，在动植物中广泛表达。最近的研究表明，circRNA广泛参与细胞增殖、细胞分化、细胞自噬等生物学过程。然而，circRNA在绵羊肌肉发育和生长中的作用和表达尚未研究。本研究首先利用RNA-seq技术对绵羊出生前后背最长肌中的circRNA进行了研究。从RNA-seq数据中总共检测到6113个circRNA。利用逆转录PCR、DNA测序和RNase R消化实验鉴定了几种circRNA。通过Real-Time RT-PCR证实了几种circRNA在产前和产后肌肉中的表达水平。对该circRNA宿主基因的GO和KEGG富集分析表明，这些circRNA主要参与肌肉生长发育相关的信号通路。这些circRNA可能在预测的circRNA-miRNA-mRNA网络中海绵化microRNA（miRNAs）。研究结果为绵羊肌肉中circRNA的研究提供了重要参考。
Circular RNAs (circRNAs), a type of non-coding RNA with circular structure, were generated by back splicing and widely expressed in animals and plants. Recent studies have shown that circRNAs extensively participate in cell proliferation, cell differentiation, cell autophagy and other biological processes. However, the role and expression of circRNAs in the development and growth of muscle have not been studied in sheep. In our study, we first used RNA-seq to study the circRNAs in prenatal and postnatal longissimus dorsi muscle of sheep. A total of 6113 circRNAs were detected from the RNA-seq data. Several circRNAs were identified using reverse transcription PCR, DNA sequencing and RNase R digestion experiments. The expression levels of several circRNAs in prenatal and postnatal muscle were confirmed by Real-Time RT-PCR. The gene ontology (GO) and KEGG enrichment analysis of the host gene of the circRNAs showed that these circRNAs were mainly involved in the growth and development of muscle related signaling pathways. These circRNAs might sponge microRNAs (miRNAs) in predicted circRNA-miRNA-mRNA networks. The circRNAs expression profiles in muscle provided an important reference for the study of circRNAs in sheep.
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影响因子：
14.9
作者：
Hansen TB;Venø MT;Damgaard CK;Kjems J
通讯作者：
Kjems J
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通讯作者：
Camper, Sally A.