A role for SPINDLY gene in the regulation of oxidative stress response in Arabidopsis
A role for SPINDLY gene in the regulation of oxidative stress response in Arabidopsis
复制标题
SPINDLY基因在拟南芥氧化应激反应调节中的作用
DOI：
10.1134/s1021443706040170
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发表时间：
2006-07
影响因子：
1.4
通讯作者：
Cao, S. Q.
中科院分区：
文献类型：
--
作者：
Ye, M.;Zhang, R. X.;Huang, Q.;Cao, S. Q.
spendly （SPY）基因编码一种假定的o-连锁n -乙酰氨基葡萄糖转移酶，酵母双杂交实验发现GIGANTEA （GI）是拟南芥中spendly相互作用的伙伴。GIGANTEA基因先前被证明参与氧化应激反应的调节；然而，SPY基因是否也参与氧化应激反应尚不清楚。在这里，我们发现SPY在氧化应激反应的调节中起作用。与野生型植物相比，spy-1突变体对百草枯（PQ）或过氧化氢（H2O2）介导的氧化胁迫更具耐受性。内源H2O2和超氧阴离子自由基以及脂质过氧化分析表明，spy-1突变体对PQ胁迫的耐受性增强不是由于PQ摄取或PQ从其作用部位隔离的缺陷，而是由于spy-1突变体减轻了PQ胁迫对植物细胞的氧化损伤。spy-1中超氧化物歧化酶（SOD）和抗坏血酸过氧化物酶（APX）组成活性较高，可能与编码叶绿体Cu/Zn SOD和APX1基因的CSD2基因激活有关。综上所述，这些结果表明，spy-1突变体对氧化应激的耐受性增强，至少在一定程度上与CSD2和APX1的组成性激活有关。
SPINDLY (SPY) gene encodes a putative O-linked N-acetyl-glucosamine transferase, and yeast two-hybrid assay identified GIGANTEA (GI) as a SPY-interacting partner in Arabidopsis. GIGANTEA gene was previously shown to be involved in the regulation of oxidative stress response; however, it is unclear whether SPY gene is also involved in oxidative stress response. Here we showed that SPY plays a role in the regulation of the oxidative stress response. The spy-1 mutant was more tolerant to paraquat (PQ)-or hydrogen peroxide (H2O2)-mediated oxidative stress than wild-type plants. Analyses of endogenous H2O2 and superoxide anion radicals as well as lipid peroxidation revealed that enhanced tolerance of the spy-1 mutant to PQ-stress was not due to defects in the PQ uptake or the PQ sequestration from its site of action but rather the spy-1 mutation alleviated oxidative damage of plant cells upon PQ stress. Higher constitutive activities of superoxide dismutase (SOD) and ascorbate peroxidase (APX) in spy-1 are more likely to be due to activation of both CSD2 gene encoding chloroplast Cu/Zn SOD and APX1 gene. Taken together, these results suggest that enhanced tolerance of the spy-1 mutant to oxidative stress is associated, at least in part, with constitutive activation of CSD2 and APX1.
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DOI：
10.1073/pnas.87.24.9903
发表时间：
1990-12
影响因子：
11.1
作者：
W. V. Camp;C. Bowler;R. Villarroel;W. Tsang;M. MONTAGUt;Dirk INZt
通讯作者：
W. V. Camp;C. Bowler;R. Villarroel;W. Tsang;M. MONTAGUt;Dirk INZt
影响因子：
7.2
作者：
Rao, MV;Davis, KR
通讯作者：
Davis, KR
影响因子：
7.2
作者：
Kurepa, J;Smalle, J;Inzé, D
通讯作者：
Inzé, D
影响因子：
4.9
作者：
Y. Nakano;K. Asada
通讯作者：
Y. Nakano;K. Asada
影响因子：
7.4
作者：
S. Storozhenko;Pascal De Pauw;M. V. Montagu;Dirk Inzé;S. Kushnir
通讯作者：
S. Storozhenko;Pascal De Pauw;M. V. Montagu;Dirk Inzé;S. Kushnir