Genome-wide identification of the class III peroxidase gene family of sugarcane and its expression profiles under stresses.
Genome-wide identification of the class III peroxidase gene family of sugarcane and its expression profiles under stresses.
复制标题
甘蔗的III类过氧化物酶基因家族及其在应力下的表达谱的全基因组鉴定。
DOI：
10.3389/fpls.2023.1101665
复制
发表时间：
2023
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
植物特异性的第三类过氧化物酶（PRXs）在木质化、细胞伸长、种子萌发以及生物和非生物胁迫中起着至关重要的作用。利用生物信息学方法和实时荧光定量PCR技术对甘蔗中的第III类过氧化物酶基因家族进行了鉴定。R570 STP中的82个PRX蛋白具有保守的PRX结构域，为III类PRX基因家族的成员。通过对甘蔗、割手密、高粱、水稻和拟南芥的系统发育分析，将ShPRX家族基因分为6类。启动子顺式作用元件的分析表明，大多数ShPRX家族基因含有涉及阿坝，MeJA，光响应，厌氧诱导，干旱诱导的顺式作用调控元件。进化分析表明，ShPRXs是在禾本科和凤梨科分化后形成的，串联重复事件在甘蔗ShPRX基因的扩增中起着重要作用。纯化选择保留了ShPRX蛋白的功能。SsPRX基因在不同生育期的茎和叶中差异表达。自发的然而，ShPRX基因在SCMV接种的甘蔗植株中差异表达。qRT-PCR分析表明，SCMV、Cd和盐胁迫均能特异性诱导甘蔗PRX基因的表达。这些结果有助于阐明甘蔗第三类PRX基因家族的结构、进化和功能，为Cd污染土壤的植物修复和抗甘蔗花叶病、盐和Cd胁迫的甘蔗新品种选育提供思路。
Plant-specific Class III peroxidases (PRXs) play a crucial role in lignification, cell elongation, seed germination, and biotic and abiotic stresses. The class III peroxidase gene family in sugarcane were identified by bioinformatics methods and realtime fluorescence quantitative PCR. Eighty-two PRX proteins were characterized with a conserved PRX domain as members of the class III PRX gene family in R570 STP. The ShPRX family genes were divided into six groups by the phylogenetic analysis of sugarcane, Saccharum spontaneum, sorghum, rice, and Arabidopsis thaliana. The analysis of promoter cis-acting elements revealed that most ShPRX family genes contained cis-acting regulatory elements involved in ABA, MeJA, light responsiveness, anaerobic induction, and drought inducibility. An evolutionary analysis indicated that ShPRXs was formed after Poaceae and Bromeliaceae diverged, and tandem duplication events played a critical role in the expansion of ShPRX genes of sugarcane. Purifying selection maintained the function of ShPRX proteins. SsPRX genes were differentially expressed in stems and leaves at different growth stages in S. spontaneum. However, ShPRX genes were differentially expressed in the SCMV-inoculated sugarcane plants. A qRT-PCR analysis showed that SCMV, Cd, and salt could specifically induce the expression of PRX genes of sugarcane. These results help elucidate the structure, evolution, and functions of the class III PRX gene family in sugarcane and provide ideas for the phytoremediation of Cd-contaminated soil and breeding new sugarcane varieties resistant to sugarcane mosaic disease, salt, and Cd stresses.
登录
查看更多内容
与干旱对比和盐度应激反应的水稻品种中与基因表达相关的不同DNA甲基化模式。
影响因子：
4.6
作者：
Garg R;Narayana Chevala V;Shankar R;Jain M
通讯作者：
Jain M
Plant-mPLoc：增强植物蛋白亚细胞定位预测能力的自上而下策略
影响因子：
3.7
作者：
Chou KC;Shen HB
通讯作者：
Shen HB
影响因子：
2.7
作者：
Chen, Yi;Feng, Jiajia;Qu, Yunfang;Zhang, Jinlong;Zhang, Li;Liang, Dong;Yang, Yujie;Huang, Jinling
通讯作者：
Huang, Jinling
影响因子：
14.9
作者：
Letunic I;Bork P
通讯作者：
Bork P
异源四倍体棉花 (Gossypium hirsutum L.) 中 III 类过氧化物酶的全基因组鉴定和分析及其对 PK 缺乏的反应
影响因子：
3.5
作者：
Duan, Pengfei;Wang, Guo;Zhang, Baohong
通讯作者：
Zhang, Baohong