Genome-wide identification and functional analysis of class III peroxidases in Gossypium hirsutum.
Genome-wide identification and functional analysis of class III peroxidases in Gossypium hirsutum.
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DOI：
10.7717/peerj.13635
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发表时间：
2022
期刊：
影响因子：
2.7
通讯作者：
Huang, Jinling
中科院分区：
文献类型：
--
作者：
Chen, Yi;Feng, Jiajia;Qu, Yunfang;Zhang, Jinlong;Zhang, Li;Liang, Dong;Yang, Yujie;Huang, Jinling
III 类过氧化物酶 (PRX) 基因在多种过程中发挥重要作用，例如生长素分解代谢、H2O2 去除、交联细胞壁成分以及对生物和非生物胁迫的响应。在本研究中，我们分别从陆地棉、植物棉和雷蒙德棉中鉴定出 166、78 和 89 个 PRX 基因。基于系统发育树分析和拟南芥中PRX基因的分类，将这些PRX基因分为7个亚科。片段复制和纯化选择是驱动GhPRXs进化的主要因素。 GO和KEGG富集分析显示GhPRX基因主要与氧化应激反应、过氧化物酶活性和苯丙素生物合成途径相关。转录组数据分析表明，不育系JinA和保持系MB177在小孢子发育过程中GhPRX基因表达存在显着差异。我们使用 qRT-PCR 证实了不育系中 GhPRX107 的上调和 GhPRX128 的下调，与其维持系相比，表明它们在花粉育性中的作用。此外，与对照相比，沉默棉花中的 GhPRX107 显示小孢子母细胞阶段花药的活性氧 (ROS) 水平显着降低。与野生型相比，拟南芥中过表达 GhPRX107 显着增加了花药的 ROS 水平。总之，我们确定 GhPRX107 是花药中 ROS 水平的决定因素。这项工作为花粉发育中的 PRX 研究奠定了基础。
Class III peroxidase (PRX) genes play essential roles in various processes, such as auxin catabolism, removal of H2O2, crosslinking cell wall components, and response to biotic and abiotic stresses. In this study, we identified 166, 78 and 89 PRX genes from G. hirsutum, G. arboretum and G. raimondii, respectively. These PRX genes were classified into seven subfamilies based on phylogenetic tree analysis and the classification of PRX genes in Arabidopsis. Segmental duplication and purifying selection were the major factors driving the evolution of GhPRXs. GO and KEGG enrichment analysis revealed that GhPRX genes were mainly associated with responding to oxidative stresses, peroxidase activities and phenylpropanoid biosynthesis pathways. Transcriptome data analysis showed that GhPRX genes expression were significantly different in microspore development between the sterility line-JinA and the maintainer line MB177. We confirmed the up-regulation of GhPRX107 and down-regulation of GhPRX128 in the sterile line compared to its maintainer line using qRT-PCR, suggesting their roles in pollen fertility. In addition, silencing GhPRX107 in cotton showed a significant decrease of the reactive oxygen species (ROS) levels of microsporocyte stage anthers compared to control. Overexpressing GhPRX107 in Arabidopsis significantly increased the ROS levels of anthers compared to wild type. In conclusion, we identified GhPRX107 as a determinant of ROS levels in anther. This work sets a foundation for PRX studies in pollen development.
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DOI：
10.1093/bioinformatics/8.3.275
发表时间：
1992-06-01
期刊：
COMPUTER APPLICATIONS IN THE BIOSCIENCES
影响因子：
--
作者：
JONES, DT;TAYLOR, WR;THORNTON, JM
通讯作者：
THORNTON, JM
DOI：
10.1093/bioinformatics/bts565
发表时间：
2012-12-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Fu L;Niu B;Zhu Z;Wu S;Li W
通讯作者：
Li W
Wolf Psort：蛋白质定位预测因子。
影响因子：
14.9
作者：
Horton, Paul;Park, Keun-Joon;Obayashi, Takeshi;Fujita, Naoya;Harada, Hajime;Adams-Collier, C J;Nakai, Kenta
通讯作者：
Nakai, Kenta
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro
影响因子：
5.6
作者：
Cao Y;Han Y;Meng D;Li D;Jin Q;Lin Y;Cai Y
通讯作者：
Cai Y