Diagnostic accuracy of Kato-Katz, FLOTAC, Baermann, and PCR methods for the detection of light-intensity hookworm and Strongyloides stercoralis infections in Tanzania.
Diagnostic accuracy of Kato-Katz, FLOTAC, Baermann, and PCR methods for the detection of light-intensity hookworm and Strongyloides stercoralis infections in Tanzania.
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DOI：
10.4269/ajtmh.13-0268
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发表时间：
2014-03
期刊：
影响因子：
--
通讯作者：
Daubenberger C
中科院分区：
文献类型：
--
作者：
Knopp S;Salim N;Schindler T;Karagiannis Voules DA;Rothen J;Lweno O;Mohammed AS;Singo R;Benninghoff M;Nsojo AA;Genton B;Daubenberger C
灵敏的诊断工具对于准确评估低流行地区的蠕虫感染至关重要。我们检查了坦桑尼亚个体的粪便样本，并将实时聚合酶链反应（PCR）的诊断准确性与FLOTAC技术和Kato-Katz方法对钩虫的诊断准确性以及Baermann方法对粪类蛔虫的检测进行了比较。只有FLOTAC法诊断钩虫的灵敏度高于Kato-Katz法；PCR法与Kato-Katz法的灵敏度相等。PCR检测粪球菌的灵敏度很低。PCR的周期阈值与钩虫卵数和粪螟幼虫数的对数呈负相关。PCR假阴性的中位幼虫数显著低于真阳性。所有方法均未能检测到非常低强度的感染。需要新的诊断方法来监测进展中的寄生虫控制规划、确认消灭或监测疾病复发。
Sensitive diagnostic tools are crucial for an accurate assessment of helminth infections in low-endemicity areas. We examined stool samples from Tanzanian individuals and compared the diagnostic accuracy of a real-time polymerase chain reaction (PCR) with the FLOTAC technique and the Kato–Katz method for hookworm and the Baermann method for Strongyloides stercoralis detection. Only FLOTAC had a higher sensitivity than the Kato–Katz method for hookworm diagnosis; the sensitivities of PCR and the Kato–Katz method were equal. PCR had a very low sensitivity for S. stercoralis detection. The cycle threshold values of the PCR were negatively correlated with the logarithm of hookworm egg and S. stercoralis larvae counts. The median larvae count was significantly lower in PCR false negatives than true positives. All methods failed to detect very low-intensity infections. New diagnostic approaches are needed for monitoring of progressing helminth control programs, confirmation of elimination, or surveillance of disease recrudescence.
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比较Kato-Katz，Koga琼脂板，以太浓度和FLOTAC的诊断精度，用于曼森和土壤传播的蠕虫。
影响因子：
3.8
作者：
Glinz, Dominik;Silue, Kigbafori D.;Knopp, Stefanie;Lohourignon, Laurent K.;Yao, Kouassi P.;Steinmann, Peter;Rinaldi, Laura;Cringoli, Giuseppe;N'Goran, Eliezer K.;Utzinger, Juerg
通讯作者：
Utzinger, Juerg
影响因子：
1.9
作者：
Habtamu, Kassahun;Degarege, Abraham;Erko, Berhanu
通讯作者：
Erko, Berhanu
影响因子：
1.9
作者：
Dendukuri, N;Joseph, L
通讯作者：
Joseph, L
影响因子：
2.6
作者：
Hawass, NED
通讯作者：
Hawass, NED
影响因子：
2.4
作者：
Brooks, SP;Gelman, A
通讯作者：
Gelman, A