Comparing diagnostic accuracy of Kato-Katz, Koga agar plate, ether-concentration, and FLOTAC for Schistosoma mansoni and soil-transmitted helminths.
Comparing diagnostic accuracy of Kato-Katz, Koga agar plate, ether-concentration, and FLOTAC for Schistosoma mansoni and soil-transmitted helminths.
复制标题
比较Kato-Katz，Koga琼脂板，以太浓度和FLOTAC的诊断精度，用于曼森和土壤传播的蠕虫。
DOI：
10.1371/journal.pntd.0000754
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发表时间：
2010-07-20
影响因子：
3.8
通讯作者：
Utzinger, Juerg
中科院分区：
文献类型：
--
作者：
Glinz, Dominik;Silue, Kigbafori D.;Knopp, Stefanie;Lohourignon, Laurent K.;Yao, Kouassi P.;Steinmann, Peter;Rinaldi, Laura;Cringoli, Giuseppe;N'Goran, Eliezer K.;Utzinger, Juerg
寄生虫和土壤传播蠕虫的感染对受影响的人口造成了相当大但未得到充分重视的经济和公共卫生负担。准确的诊断对于患者管理、药物疗效评估和大规模社区控制项目的监测至关重要。四种粪便显微镜技术的诊断准确性（即，Kato-Katz、古贺琼脂平板、乙醚浓度和FLOTAC）检测曼氏血吸虫和土传蠕虫卵的方法进行了比较。所有四种方法的综合结果作为诊断的“金”标准，并揭示了S。曼氏线虫、钩虫、鞭虫、粪类圆线虫和蛔虫的检出率分别为83.0%、55.4%、40.2%、33.9%和28.6%。在醋酸钠-醋酸-福尔马林中保存30天或83天的粪便样品的单个FLOTAC显示对S.粪便标本保存40天后乙醚浓缩法（85.0%）和Kato-Katz法（77.4%）对曼氏病的诊断准确率分别为91.4%和100%。此外，一个单一的FLOTAC检测钩虫，A。蚯蚓和T.鞭毛虫感染的敏感性比任何其他方法使用，但导致较低的鸡蛋计数。古贺琼脂平板法是最准确的诊断方法。粪虫我们已经表明，FLOTAC方法有希望为诊断S。mansoni此外，我们的研究证实，FLOTAC是一种敏感的技术，用于检测常见的土壤传播的蠕虫。对S.粪生菌，古贺琼脂平板法仍然是选择的方法。寄生虫蠕虫（例如，曼氏血吸虫、钩虫、蛔虫、鞭虫和线虫）在发展中国家仍然广泛存在。准确的诊断对于更好地管理患者和监测驱虫程序非常重要。不幸的是，检测粪便样本中寄生虫卵或幼虫的方法缺乏灵敏度，特别是当感染强度较低时。目前应用最广泛的诊断方法是S。曼氏线虫、钩虫、蛔虫和鞭虫的流行病学调查是Kato-Katz技术。最近，FLOTAC技术在诊断钩虫、蛔虫和鞭虫方面显示出比Kato-Katz方法更高的灵敏度，但没有关于S. mansoni我们比较了FLOTAC与Kato-Katz、乙醚浓缩和古贺琼脂平板技术对沙门氏菌的诊断准确性。使用科特迪瓦112名学童的粪便样本，对曼索尼和其他寄生虫感染进行了研究。FLOTAC对S.曼氏诊断然而，当使用FLOTAC时，鸡蛋计数较低，这是一个需要进一步调查的问题。FLOTAC、Kato-Katz和乙醚浓缩技术未能准确检测线虫幼虫，因此，古贺琼脂平板仍然是这种被忽视的寄生虫的首选方法。
Infections with schistosomes and soil-transmitted helminths exert a considerable yet underappreciated economic and public health burden on afflicted populations. Accurate diagnosis is crucial for patient management, drug efficacy evaluations, and monitoring of large-scale community-based control programs. The diagnostic accuracy of four copromicroscopic techniques (i.e., Kato-Katz, Koga agar plate, ether-concentration, and FLOTAC) for the detection of Schistosoma mansoni and soil-transmitted helminth eggs was compared using stool samples from 112 school children in Côte d'Ivoire. Combined results of all four methods served as a diagnostic ‘gold’ standard and revealed prevalences of S. mansoni, hookworm, Trichuris trichiura, Strongyloides stercoralis and Ascaris lumbricoides of 83.0%, 55.4%, 40.2%, 33.9% and 28.6%, respectively. A single FLOTAC from stool samples preserved in sodium acetate-acetic acid-formalin for 30 or 83 days showed a higher sensitivity for S. mansoni diagnosis (91.4%) than the ether-concentration method on stool samples preserved for 40 days (85.0%) or triplicate Kato-Katz using fresh stool samples (77.4%). Moreover, a single FLOTAC detected hookworm, A. lumbricoides and T. trichiura infections with a higher sensitivity than any of the other methods used, but resulted in lower egg counts. The Koga agar plate method was the most accurate diagnostic assay for S. stercoralis. We have shown that the FLOTAC method holds promise for the diagnosis of S. mansoni. Moreover, our study confirms that FLOTAC is a sensitive technique for detection of common soil-transmitted helminths. For the diagnosis of S. stercoralis, the Koga agar plate method remains the method of choice. Infections with parasitic worms (e.g., Schistosoma mansoni, hookworm, roundworm, whipworm, and threadworm) are still widespread in the developing world. Accurate diagnosis is important for better patient management and for monitoring of deworming programs. Unfortunately, methods to detect parasite eggs or larvae in stool samples lack sensitivity, particularly when infection intensities are low. The most widely used method for the diagnosis of S. mansoni, hookworm, roundworm and whipworm in epidemiological surveys is the Kato-Katz technique. Recently, the FLOTAC technique has shown a higher sensitivity than the Kato-Katz method for the diagnosis of hookworm, roundworm and whipworm, but no data are available for S. mansoni. We compared the diagnostic accuracy of the FLOTAC with the Kato-Katz, ether-concentration and Koga agar plate techniques for S. mansoni and other parasitic worm infections using stool samples from 112 school children from Côte d'Ivoire. FLOTAC showed the highest sensitivity for S. mansoni diagnosis. Egg counts, however, were lower when using FLOTAC, an issue which needs further investigations. The FLOTAC, Kato-Katz and ether-concentration techniques failed to accurately detect threadworm larvae, and hence, the Koga agar plate remains the method of choice for this neglected parasite.
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影响因子：
4.9
作者：
Brooker, Simon;Kabatereine, Narcis B.;Snow, Robert W.
通讯作者：
Snow, Robert W.
DOI：
10.1016/j.trstmh.2009.05.012
发表时间：
2009-12-01
影响因子：
2.2
作者：
Knopp, Stefanie;Glinz, Dominik;Utzinger, Juerg
通讯作者：
Utzinger, Juerg
DOI：
10.1016/0169-4758(92)90144-q
发表时间：
1992-08-01
期刊：
PARASITOLOGY TODAY
影响因子：
--
作者：
DEVLAS, SJ;GRYSEELS, B
通讯作者：
GRYSEELS, B
DOI：
10.4269/ajtmh.2008.78.140
发表时间：
2008-01-01
影响因子：
3.3
作者：
Enk, Martin Johannes;Lustosa Lima, Anna Carolina;Schall, Virginia Torres
通讯作者：
Schall, Virginia Torres
影响因子：
2.7
作者：
King, Charles H.
通讯作者：
King, Charles H.