The chromodomain-containing NH(2)-terminus of Chromator interacts with histone H1 and is required for correct targeting to chromatin.
The chromodomain-containing NH(2)-terminus of Chromator interacts with histone H1 and is required for correct targeting to chromatin.
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DOI：
10.1007/s00412-011-0355-4
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发表时间：
2012-04
期刊：
影响因子：
1.6
通讯作者：
Johansen J
中科院分区：
文献类型：
--
作者：
Yao C;Ding Y;Cai W;Wang C;Girton J;Johansen KM;Johansen J
染色体结构域蛋白质Chromator可分为两个主要结构域，即含有染色体结构域（ChD）的NH 2末端结构域（NTD）和含有核定位信号的COOH末端结构域（CTD）。在间期，染色质定位于染色体;然而，在细胞分裂期间，染色质重新分布以与其他核蛋白一起形成大分子纺锤体基质复合物，其有助于微管纺锤体动力学和有丝分裂期间的适当染色体分离。先前已经证明，CTD足以将Chromirons靶向纺锤体基质。在这项研究中，我们表明，NTD结构域的染色质是必需的适当的本地化染色质在间期和染色体形态缺陷中观察到的染色质亚纯型突变体的背景下，可以在很大程度上拯救这个域的表达。此外，我们表明，ChD结构域可以与组蛋白H1相互作用，这种相互作用是必要的正确的染色质靶向。尽管如此，在ChD不存在的情况下，染色质的定位仍然发生，这表明染色质结合具有第二种机制，我们提供的证据表明，这种结合是由NTD中的其他序列介导的。总之，这些研究结果表明，染色质的染色质功能主要是由NH 2-末端结构域，而有丝分裂相关的功能主要是由COOH-末端序列介导的。
The chromodomain protein, Chromator, can be divided into two main domains, a NH2- terminal domain (NTD) containing the chromodomain (ChD) and a COOH-terminal domain (CTD) containing a nuclear localization signal. During interphase Chromator is localized to chromosomes; however, during cell division Chromator redistributes to form a macro molecular spindle matrix complex together with other nuclear proteins that contribute to microtubule spindle dynamics and proper chromosome segregation during mitosis. It has previously been demonstrated that the CTD is sufficient for targeting Chromator to the spindle matrix. In this study we show that the NTD domain of Chromator is required for proper localization to chromatin during interphase and that chromosome morphology defects observed in Chromator hypomorphic mutant backgrounds can be largely rescued by expression of this domain. Furthermore, we show that the ChD domain can interact with histone H1 and that this interaction is necessary for correct chromatin targeting. Nonetheless, that localization to chromatin still occurs in the absence of the ChD indicates that Chromator possesses a second mechanism for chromatin association and we provide evidence that this association is mediated by other sequences residing in the NTD. Taken together these findings suggest that Chromator's chromatin functions are largely governed by the NH2-terminal domain whereas functions related to mitosis are mediated mainly by COOH-terminal sequences.
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DOI：
10.3791/1748
发表时间：
2010-02-09
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Cai, Weili;Jin, Ye;Johansen, Kristen M
通讯作者：
Johansen, Kristen M
影响因子：
16
作者：
Nielsen, AL;Oulad-Abdelghani, M;Losson, R
通讯作者：
Losson, R
影响因子：
14.8
作者：
通讯作者：
--
影响因子：
1.6
作者：
Gortchakov, AA;Eggert, H;Saumweber, H
通讯作者：
Saumweber, H
影响因子：
11.4
作者：
Brasher, SV;Smith, BO;Laue, ED
通讯作者：
Laue, ED