A c-di-GMP binding effector controls cell size in a cyanobacterium.
A c-di-GMP binding effector controls cell size in a cyanobacterium.
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DOI：
10.1073/pnas.2221874120
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发表时间：
2023-03-28
影响因子：
11.1
通讯作者：
Zhang, Cheng-Cai
中科院分区：
文献类型：
--
作者：
Zeng, Xiaoli;Huang, Min;Sun, Qing-Xue;Peng, Ye-Jun;Xu, Xiaomei;Tang, Yun-Bin;Zhang, Ju-Yuan;Yang, Yiling;Zhang, Cheng-Cai
细胞大小的稳态是细菌的一个重要物理特性，如何维持和调节细胞大小一直是微生物学中的一个重要问题。我们最近发现，第二信使c-di-GMP可能是鱼腥藻细胞大小控制的细胞内代理。在这项研究中，我们确定了一个高度保守的c-di-GMP受体在蓝藻和证明，它调节细胞大小通过相互作用与转录因子DevH鱼腥藻。这些发现不仅提供了细胞大小调节的机制，而且还扩展了c-di-GMP受体在细菌中的作用。由于c-di-GMP在蓝藻中发挥着广泛的功能，我们的开创性研究构成了这些生物体中c-di-GMP信号转导研究的基础。Cyclic-di-GMP（c-di-GMP）是一种普遍存在的细菌信号分子。它也是调节细胞大小和细胞行为的关键参与者，如蓝细菌中的细胞聚集和趋光性，蓝细菌因其在地球生态和进化中的作用而构成了原核生物的重要群体。然而，c-di-GMP受体从未在蓝藻中发现。在这里，我们报告的C-二-GMP受体，CdgR，从丝状蓝藻鱼腥藻PCC 7120的鉴定。晶体结构分析和遗传学研究表明，CdgR在二聚体界面结合c-di-GMP，这种结合是以c-di-GMP依赖性方式控制细胞大小所必需的。CdgR在配体结合和信号传递中的不同功能可以在遗传上分开，使我们能够剖析其分子信号传导功能。CdgR的脱辅基形式的存在触发细胞尺寸减小，与在细胞中c-di-GMP水平降低的情况下观察到的类似作用一致。此外，我们发现CdgR通过与全局转录因子DevH相互作用来发挥其功能，并且这种相互作用被c-di-GMP抑制。由DevH的条件性耗尽或由细胞中CdgR的几个点突变蛋白的产生所触发的致死效应表明，该信号传导途径在鱼腥藻中起着关键作用。我们的研究揭示了c-di-GMP信号传导在控制细胞大小中的机制，细胞大小是细菌的一个重要而复杂的性状。CdgR在蓝藻中是高度保守的，这将极大地扩展我们对c-di-GMP信号在这些生物中的作用的理解。
Cell size homeostasis is an essential physical trait of bacteria, and how the cell size is maintained and regulated remains an important question in microbiology. We recently found that the second messenger c-di-GMP may be an intracellular proxy for cell size control in Anabaena. In this study, we identified and characterized a highly conserved c-di-GMP receptor in cyanobacteria and demonstrated that it regulates cell size via interaction with the transcription factor DevH in Anabaena. These findings not only provide a mechanism of cell size regulation but also extend the role of c-di-GMP receptors in bacteria. Since c-di-GMP plays broad functions in cyanobacteria, our pioneering study constitutes a basis for studies on c-di-GMP signaling in these organisms. Cyclic-di-GMP (c-di-GMP) is a ubiquitous bacterial signaling molecule. It is also a critical player in the regulation of cell size and cell behaviors such as cell aggregation and phototaxis in cyanobacteria, which constitute an important group of prokaryotes for their roles in the ecology and evolution of the Earth. However, c-di-GMP receptors have never been revealed in cyanobacteria. Here, we report the identification of a c-di-GMP receptor, CdgR, from the filamentous cyanobacterium Anabaena PCC 7120. Crystal structural analysis and genetic studies demonstrate that CdgR binds c-di-GMP at the dimer interface and this binding is required for the control of cell size in a c-di-GMP-dependent manner. Different functions of CdgR, in ligand binding and signal transmission, could be separated genetically, allowing us to dissect its molecular signaling functions. The presence of the apo-form of CdgR triggers cell size reduction, consistent with the similar effects observed with a decrease of c-di-GMP levels in cells. Furthermore, we found that CdgR exerts its function by interacting with a global transcription factor DevH, and this interaction was inhibited by c-di-GMP. The lethal effect triggered by conditional depletion of DevH or by the production of several point-mutant proteins of CdgR in cells indicates that this signaling pathway plays critical functions in Anabaena. Our studies revealed a mechanism of c-di-GMP signaling in the control of cell size, an important and complex trait for bacteria. CdgR is highly conserved in cyanobacteria, which will greatly expand our understanding of the roles of c-di-GMP signaling in these organisms.
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c-di-GMP 稳态对于鱼腥藻异囊发育至关重要。
影响因子：
5.2
作者：
Huang M;Zhang JY;Zeng X;Zhang CC
通讯作者：
Zhang CC
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH
影响因子：
4.7
作者：
Niu, Tian-Cai;Lin, Gui-Ming;Zhang, Cheng-Cai
通讯作者：
Zhang, Cheng-Cai
影响因子：
7.3
作者：
Hengge, Regine
通讯作者：
Hengge, Regine