Human immune responses to hapten-conjugated cells. I. Primary and secondary proliferative responses in vitro.
Human immune responses to hapten-conjugated cells. I. Primary and secondary proliferative responses in vitro.
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DOI：
10.1084/jem.147.6.1671
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发表时间：
1978-06-01
期刊：
影响因子：
--
通讯作者：
Rich RR
中科院分区：
文献类型：
--
作者：
Seldin MF;Rich RR
建立了人淋巴细胞对半抗原结合的外周血单核细胞的原代和继发性增殖反应的体外模型。人淋巴细胞与自体三硝基苯基(TNP)偶联的刺激细胞共培养可产生原代增殖反应。受试者分为高反应者和低反应者，平均刺激指数分别为11和2.1。在初始致敏后3wk，高应答受试者的预刺激细胞产生二次增殖反应。为了研究二次刺激的抗原性要求，用自体和异体TNP偶联刺激物对自体TNP偶联预激反应者进行了再刺激。在所有实验中，使用自体结合细胞的再刺激比使用同种异体结合细胞产生的增殖反应要大得多。然后进行实验，以确定预置反应者和刺激细胞之间的HLA决定簇同源性是否影响二次反应性的水平。人类白细胞抗原-A和B位点的血清学决定因素的同源性与增强的反应性无关。相反，B细胞抗原分型检测到的D区决定簇的同源性与二次应答的大小呈高度显著的正相关。因此，这些数据有力地表明，对于TNP的次级增殖反应，人类T细胞识别与人类白细胞抗原-D区决定因素相关的半抗原。
An in vitro model was developed to study both primary and secondary proliferative responses of human lymphocytes to hapten-conjugated peripheral blood mononuclear cells. Coculture of human lymphocytes with autologous trinitrophenyl (TNP)-conjugated stimulator cells resulted in primary proliferative responses. Subjects segregated into high and low primary responders with mean stimulation indices of 11 and 2.1, respectively. Restimulation of primed cells from high responder subjects 3 wk after initial sensitization generated secondary proliferative responses. To investigate the antigenic requirements for secondary stimulation, autologous TNP-conjugate primed responders were restimulated with both autologous and allogeneic TNP-conjugated stimulators. In all experiments restimulation with autologous conjugated cells yielded substantially greater proliferative responses than with allogeneic conjugates. Experiments were then performed to ascertain whether HLA determinant homology between primed responder and stimulator cells influenced the level of secondary responsiveness. Homology for HLA-A and B locus serologic determinants was not associated with enhanced responsiveness. In contrast, D region determinant homology, detected by B-cell antigen typing, showed a highly significant positive correlation with the magnitude of secondary responses. The data thus strongly suggest that for secondary proliferative responses to TNP, human T cells recognize hapten in association with HLA-D region determinants.
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影响因子：
15.9
作者：
BARBOSA, J;KING, R;YUNIS, EJ
通讯作者：
YUNIS, EJ
影响因子：
64.8
作者：
DICKMEISS, E;SOEBERG, B;SVEJGAARD, A
通讯作者：
SVEJGAARD, A
影响因子：
3.2
作者：
FORMAN, J;KLEIN, J
通讯作者：
KLEIN, J
DOI：
10.1084/jem.142.6.1349
发表时间：
1975-12-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Bevan MJ
通讯作者：
Bevan MJ
影响因子：
--
作者：
McDevitt, H O;Benacerraf, B
通讯作者：
Benacerraf, B