Total lymphocyte deficiency attenuates AngII-induced atherosclerosis in males but not abdominal aortic aneurysms in apoE deficient mice.
Total lymphocyte deficiency attenuates AngII-induced atherosclerosis in males but not abdominal aortic aneurysms in apoE deficient mice.
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DOI：
10.1016/j.atherosclerosis.2010.02.034
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发表时间：
2010-08
期刊：
影响因子：
5.3
通讯作者：
Daugherty A
中科院分区：
文献类型：
--
作者：
Uchida HA;Kristo F;Rateri DL;Lu H;Charnigo R;Cassis LA;Daugherty A
T淋巴细胞和B淋巴细胞与动脉粥样硬化和动脉瘤有关。血管紧张素II （AngII）输注到高胆固醇血症小鼠体内，导致动脉粥样硬化和腹主动脉瘤（AAA）形成增加。在这项研究中，我们确定了总淋巴细胞缺乏是否会减少血管病变。将ApoE缺陷（ApoE−/−）小鼠与缺乏成熟T淋巴细胞和B淋巴细胞的重组激活基因-1 （rag1）缺陷小鼠杂交。杂合子幼崽（rag1 +/−）淋巴细胞功能正常，作为对照。rag1 +/−或- /−的apoE−/−雄性和雌性小鼠喂食正常实验室饮食，并通过渗透微型泵皮下注射生理盐水或AngII (1,000 ng/kg/min)，持续28天。在AngII输注前和输注期间，总淋巴细胞缺乏对体重和收缩压没有显著影响。然而，它与血清胆固醇浓度降低有关。与生理盐水相比，AngII输注使Rag-1 +/−小鼠的动脉粥样硬化病变面积增加（雄性P = 0.017，雌性P = 0.004）。这种效应在rag1−/−雄性小鼠中明显减弱（P = 0.044），但在雌性小鼠中没有减弱。血管灌注促进了腹主动脉宽度的增加，在男性中效果更大。尽管男性动脉粥样硬化发生率降低，但无论男女，rag1缺乏对血管i诱导的主动脉扩张均无显著影响。T和B淋巴细胞缺乏可减弱雄性apoE−/−小鼠血管粥样硬化的形成，但不能减弱AAA的形成。
T and B lymphocytes are associated with atherosclerosis and aneurysms. Angiotensin II (AngII) infusion into hypercholesterolemic mice results in augmentation of atherosclerosis and abdominal aortic aneurysm (AAA) formation. In this study, we determined whether total lymphocyte deficiency reduced AngII-induced vascular diseases. ApoE deficient (apoE −/−) mice were cross-bred with recombination activating gene-1 (Rag-1) deficient mice that lack mature T and B lymphocytes. Heterozygous littermates (Rag-1 +/−) have normal lymphocytic function and served as controls. Male and female apoE −/− mice that were either Rag-1 +/− or −/− were fed a normal laboratory diet and infused with either saline or AngII (1,000 ng/kg/min) subcutaneously via osmotic mini pump for 28 days. Total lymphocyte deficiency had no significant effect on body weight and systolic blood pressure prior to and during AngII infusion. However, it was associated with decreased serum cholesterol concentrations. AngII infusion increased atherosclerotic lesion area in Rag-1 +/− mice compared to saline (P = 0.017 in males and P = 0.004 in females). This effect was significantly blunted in Rag-1 −/− male (P = 0.044), but not female mice. AngII-infusion promoted increased width of the abdominal aorta, with a greater effect in males. Despite the reduction in atherosclerosis in males, Rag-1 deficiency had no significant effect on AngII-induced aortic dilation in either gender. T and B lymphocyte deficiency attenuates AngII-induced atherosclerosis in males but not AAA formation in apoE −/− mice.
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影响因子：
15.9
作者：
Song, L;Leung, C;Schindler, C
通讯作者：
Schindler, C
影响因子：
15.9
作者：
Bruemmer, D;Collins, AR;Hsueh, WA
通讯作者：
Hsueh, WA
影响因子：
37.8
作者：
Thomas, Manesh;Gavrila, Dan;Weintraub, Neal L.
通讯作者：
Weintraub, Neal L.
DOI：
10.1161/01.atv.17.11.2333
发表时间：
1997-11-01
影响因子：
8.7
作者：
Bonthu, S;Heistad, DD;Faraci, FM
通讯作者：
Faraci, FM
影响因子：
37.8
作者：
King, Victoria L.;Lin, Alexander Y.;Gerszten, Robert E.
通讯作者：
Gerszten, Robert E.