Differential expression of serotonin 5-HT2 receptors during rat embryogenesis.
Differential expression of serotonin 5-HT2 receptors during rat embryogenesis.
复制标题
大鼠胚胎发生过程中血清素 5-HT2 受体的差异表达。
DOI：
10.1159/000017362
复制
发表时间：
1999
影响因子：
2.9
通讯作者：
Singh,S
中科院分区：
文献类型：
--
作者：
Wu,C;Dias,P;Kumar,S;Lauder,JM;Singh,S
5-羟色胺(5-羟色胺)是一种神经递质，也是一种激素和生长因子。5-羟色胺参与多种生理活动，具有复杂的药理作用。作为一种生长因子，5-羟色胺在神经性和非神经性组织的细胞增殖和分化中发挥作用，它通过14种以上亚型的5-羟色胺受体传递信号。由于确定5-羟色胺受体的表达和分布对于了解5-羟色胺受体的作用很重要，我们建立了一种核糖核酸酶保护试验(RPA)，允许同时分析每个RNA样本的5-HT2AR、5-HT2BR和5-HT2CR。这个多探针组还包括两个看家基因L32和GAPDH的探针，这两个基因控制样本加载错误。利用这套RPA探针，我们检测了5-HT2AR、5-HT2BR和5-HT2CR在胚胎第9天到21天胚胎中的相对表达。我们的数据表明，从ED11到ED21，5-HT2AR水平逐渐升高。5-HT2BR的表达在ED9~ED11之间呈下降趋势，到ED21后保持相对稳定。5-HT2CR最初在ED9和ED12之间的残留水平表达，但在ED13急剧增加到峰值，然后在ED17下降。RT-PCR证实5-HT2受体在这些组织中的表达是独立的，表明这些受体的表达存在发育调节。5-HT2R多探针分析将有助于检测这些受体在发育、正常和病理组织中表达的相对变化，以及监测因使用药物而导致的表达相对变化。
Serotonin (5-HT) is a neurotransmitter that also functions as a hormone and a growth factor. 5-HT is involved in numerous physiological actions and displays complex pharmacological properties. As a growth factor, 5-HT plays a role in cell proliferation and differentiation of neuronal and nonneuronal tissue and it transduces its signals through more than fourteen subtypes of 5-HT receptors. Since determination of the expression and distribution is important for understanding the role of the 5-HT receptors, we have developed an RNase protection assay (RPA) that allows the simultaneous analysis of 5-HT2AR, 5-HT2BR, and 5-HT2CR per sample of RNA. This multiprobe set also comprises probes for two house-keeping genes, L32 and GAPDH, which control for sample-loading errors. Using this RPA probe set, we have examined the relative expression of 5-HT2AR, 5-HT2BR, and 5-HT2CR in rat embryos inclusively from embryonic day (ED) 9 to 21 of development. Our data indicate that 5-HT2AR levels gradually increased from ED11 to ED21. The expression of 5-HT2BR was decreased between ED9 to ED11 then remained relatively constant through ED21. 5-HT2CR was initially expressed at residual levels between ED9 and ED12 but dramatically increased to a peak level at ED13, then decreased by ED17. Expression of the 5-HT2receptors in these tissues was confirmed independently by RT-PCR indicating that there is developmental regulation in the expression of these receptors. The 5-HT2R multiprobe assay will be useful for detecting relative changes in the expression of these receptors in developmental, normal and pathological tissues as well as for monitoring relative changes in expression resulting from the use of pharmaceutical agents.
登录
查看更多内容
昂丹司琼对中度阻塞性睡眠呼吸暂停的影响，一晚安慰剂对照试验
影响因子：
4.4
作者：
J. Stradling;Debbie Smith;M. Radulovacki;D. Carley
通讯作者：
D. Carley
影响因子：
158.5
作者：
YOUNG, T;PALTA, M;BADR, S
通讯作者：
BADR, S
影响因子：
5
作者：
Punjabi, NM;Shahar, E;Resnick, HE
通讯作者：
Resnick, HE
神经毒素引起的脑干损伤对清醒大鼠呼吸反应的影响
DOI：
10.1111/j.1440-1681.1985.tb00890.x
发表时间：
1985
影响因子：
2.9
作者：
R. L. Martin;H. R. Grundy
通讯作者：
H. R. Grundy
正常睡眠期间和快速眼动睡眠剥夺后髓质网状结构中的细胞外血清素水平
影响因子：
2.9
作者：
C. Blanco;R. Salín
通讯作者：
R. Salín