Reciprocal Consomic Strains to Evaluate Y Chromosome Effects
Reciprocal Consomic Strains to Evaluate Y Chromosome Effects
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用于评估 Y 染色体效应的互惠体菌株
DOI：
10.1161/01.hyp.37.2.391
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
A. Dominiczak
中科院分区：
文献类型：
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作者：
C. Negrín;M. McBride;Hillary V.O. Carswell;D. Graham;F. Carr;J. Clark;B. Jeffs;N. Anderson;I. Macrae;A. Dominiczak
我们以前已经证明，SHRSP Y染色体含有一个位点，有助于高血压的SHRSP/WKY F2杂种和SHRSP表现出局灶性脑缺血后永久性大脑中动脉闭塞（MCAO）的脆弱性增加。这种增加的脆弱性是作为一个共显性性状遗传的，Y染色体的一个假定的作用已被建议在F1杂种。本研究旨在进一步探讨Y染色体在血压调节和脑缺血易感性中的作用。我们通过选择性地将WKY大鼠的Y染色体替换为SHRSP的Y染色体，反之亦然，通过使用标记辅助育种策略，构建了同源品系。永久性MCAO通过电凝进行，梗死体积表示为同侧半球的百分比。在5周的基线期内通过无线电遥测测量收缩压，随后进行3周的盐负荷期。我们观察到，Y染色体从WKY转移到SHRSP背景显著降低了盐负荷期间的收缩压（209.2±10.4 mm Hg对241.7±7.7 mm Hg，F=5.88，P =0.038）。WKY、SPGlaYs（n=5）与亲本WKY（n=6）相比，收缩压升高（147.6± 2.4mmHg对132.6± 5.1mmHg，F=6.11，P =0.035）。同源体菌株的细胞体积与其各自的亲本菌株无显著差异：WKY.SPGlaYs（n=7）与WKY（n=7），22.8±3.7%与22.2± 8.0%，95%CI =-12.7，4.2，P =0.3; SP.WKYGlaYw（n=7）与SHRSP（n=6），37.7±4.4%与33.6± 7.6%，95% CI=−20.3，12.1，P =0.5。我们的结论是，SHRSP Y染色体窝藏一个位点有助于收缩压，而没有贡献的脆弱性脑缺血可以检测到。
We have previously demonstrated that the SHRSP Y chromosome contains a locus that contributes to hypertension in SHRSP/WKY F2 hybrids and that SHRSP exhibit an increased vulnerability to focal cerebral ischemia after permanent middle cerebral artery occlusion (MCAO). This increased vulnerability is inherited as a codominant trait, and a putative role for the Y chromosome has been suggested in F1 hybrids. The objective of this study was to investigate further the role of Y chromosome in blood pressure (BP) regulation and in the vulnerability to cerebral ischemia. We have constructed consomic strains by selectively replacing the Y chromosome from WKY rats with that of SHRSP, and vice versa, by using a marker-assisted breeding strategy. Permanent MCAO was carried out by electrocoagulation, with infarct volume expressed as a percentage of the ipsilateral hemisphere. Systolic blood pressure was measured by radiotelemetry during a baseline period of 5 weeks followed by a 3-week period of salt loading. We observed that the transfer of the Y chromosome from WKY onto SHRSP background significantly reduced systolic BP in consomic strains, SP.WKYGlaYw (n=6) versus SHRSP (n=6) (209.2±10.4 mm Hg versus 241.7±7.7 mm Hg, F=5.88, P =0.038) during the salt-loading period. In the reciprocal consomic strain, WKY.SPGlaYs (n=5), systolic BP was increased compared with WKY parental strain (n=6) (147.6±2.4 mm Hg versus 132.6±5.1 mm Hg, F=6.11, P =0.035) during baseline. Infarct volumes in consomic strains were not significantly different from their respective parental strain: WKY.SPGlaYs (n=7) versus WKY (n=7), 22.8±3.7% versus 22.2±8.0%, 95% CI=−12.7, 4.2, P =0.3; SP.WKYGlaYw (n=7) versus SHRSP (n=6), 37.7±4.4% versus 33.6±7.6%, 95% CI=−20.3, 12.1, P =0.5. We conclude that the SHRSP Y chromosome harbors a locus contributing to systolic BP, whereas no contribution to vulnerability to cerebral ischemia can be detected.
睾酮对不同 Y 染色体大鼠肾脏去甲肾上腺素含量和释放的影响。
DOI：
10.1161/01.hyp.32.5.880
发表时间：
1998
期刊：
Hypertension (Dallas, Tex. : 1979)
影响因子：
--
作者：
Jones,TJ;Dunphy,G;Milsted,A;Ely,D
通讯作者：
Ely,D
自发性高血压大鼠的昼夜血压变化和膳食盐。
DOI：
10.1161/01.hyp.24.1.1
发表时间：
1994
期刊：
Hypertension (Dallas, Tex. : 1979)
影响因子：
--
作者：
Calhoun,DA;Zhu,S;Wyss,JM;Oparil,S
通讯作者：
Oparil,S