Identification of wysPII as an Activator of Morphological Development in Streptomyces albulus CK-15.
Identification of wysPII as an Activator of Morphological Development in Streptomyces albulus CK-15.
复制标题
鉴定 wysPII 作为白色链霉菌 CK-15 形态发育激活剂
DOI：
10.3389/fmicb.2018.02550
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发表时间：
2018
影响因子：
5.2
通讯作者：
Zhang K
中科院分区：
文献类型：
--
作者：
Liu B;Ge B;Ma J;Wei Q;Khan AA;Shi L;Zhang K
武夷菌素是由白色链霉菌（Streptomyces albulus var.在农业上广泛用于防治黄瓜霜霉病、草莓白粉病、番茄灰霉病等多种真菌病害。作为一种工业化生产的生物农药，降低生产成本对于该方法的推广非常重要。为了获得快速生长的菌株，有效地缩短发酵时间，我们研究了在S. albulus菌株CK-15。ΔwysPII突变体表现出生长和孢子形成速率降低。与野生型（WT）菌株相比，ooPII（wysPII过表达）菌株中达到最大菌丝体生物量所需的时间缩短了约18小时。此外，在ooPII菌株中达到最大武夷菌素产量的时间为56小时，而在WT菌株中为62小时。此外，wysPII被证明是作为一个激活剂的形态发育，而不影响武夷菌素生产。因此，ooPII菌株可用于降低武夷菌素生产的工业发酵过程中的成本并提高效率。
Wuyiencin is produced by Streptomyces albulus var. wuyiensis and used widely in agriculture to control a variety of fungal diseases, such as cucumber downy mildew, strawberry powdery mildew, and tomato gray mold. As an industrially-produced biopesticide, reducing production costs is very important for popularization of this approach. To obtain a rapidly growing strain that effectively shortens the fermentation time, we investigated the effects of knockout and overexpression of the wysPII gene, a member of the LuxR regulatory gene family, in S. albulus strain CK-15. The ΔwysPII mutant exhibited a reduced rate of growth and sporulation. The time taken to reach the greatest mycelial biomass was approximately 18 h shorter in the ooPII (wysPII overexpressing) strain compared with that of the wild-type (WT) strain. In addition, the time to reach the greatest wuyiencin production was 56 h in the ooPII strain compared with 62 h in the WT strain. Furthermore, wysPII was shown to act as an activator of morphological development without affecting wuyiencin production. Thus, the ooPII strain can be used to reduce costs and increase efficiency in industrial fermentation processes for wuyiencin production.
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影响因子：
2.8
作者：
He, Weiqing;Lei, Jian;Wang, Yiguang
通讯作者：
Wang, Yiguang
影响因子：
5.6
作者：
Ducros, VMA;Lewis, RJ;Brannigan, JA
通讯作者：
Brannigan, JA
影响因子：
5.6
作者：
Birck, C;Malfois, M;Samama, JP
通讯作者：
Samama, JP
影响因子：
3.2
作者：
Alonso-Hearn, Marta;Eckstein, Torsten M.;Bermudez, Luiz E.
通讯作者：
Bermudez, Luiz E.
DOI：
10.1099/00221287-143-5-1503
发表时间：
1997-05-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
Labes, G;Bibb, M;Wohlleben, W
通讯作者：
Wohlleben, W