The dictyostelium kinome--analysis of the protein kinases from a simple model organism.
The dictyostelium kinome--analysis of the protein kinases from a simple model organism.
复制标题
DOI：
10.1371/journal.pgen.0020038
复制
发表时间：
2006-03
期刊：
影响因子：
4.5
通讯作者：
Smith, Janet L.
中科院分区：
文献类型：
--
作者：
Goldberg, Jonathan M.;Manning, Gerard;Liu, Allen;Fey, Petra;Pilcher, Karen E.;Xu, Yanji;Smith, Janet L.
盘基网骨藻是一种广泛研究的模式生物，在其发育周期中具有单细胞和多细胞形式。网骨藻基因组编码285种预测的蛋白激酶，与更高级的果蝇的数量相似。它包含真菌和后生动物共有的大多数激酶类的成员，以及许多以前被认为是后生动物特异性的，表明它们已经从真菌谱系中二次丢失。这包括整个酪氨酸激酶样（TKL）组，其在网骨藻中扩展并包括几种新的受体激酶。Dictyosteoblasts缺乏酪氨酸激酶组激酶，大多数酪氨酸磷酸化似乎是由TKL激酶介导的。大约一半的网囊藻激酶出现在不存在于酵母或后生动物中的亚科中，这表明蛋白激酶在网囊藻适应其栖息地中发挥了关键作用。这项研究提供了深入了解激酶的演变，并提供了一个重点，在这个系统中的信号分析。蛋白激酶是参与细胞通讯途径的真核酶，并从细胞外或细胞内的亚细胞组分之间传递信息。大约2.5%的基因编码蛋白激酶，其中许多基因的突变会导致人类疾病。作者描述了来自Dictyosteelium discoideum的全套蛋白激酶（激酶组），Dictyosteelium discoideum是一种社会性阿米巴，通过形成细胞聚集体来响应饥饿，然后分化成多细胞子实体。网骨藻在植物之后但在真菌之前从脊椎动物谱系中分支出来，因此阐明了进化史上一个有趣的时期。通过将Dictyosteoblasts激酶组与其他生物体的激酶组进行比较，作者发现了46种似乎在所有生物体中保守的激酶，并且可能参与基本的细胞过程。网骨藻是一种已建立的模式生物，用于研究人类中保守的细胞生物学的许多方面，对保守激酶的阐述将有助于指导未来的研究。网骨藻激酶组也包含了令人印象深刻的创造性程度-几乎一半的激酶是唯一的网骨藻。这些Dictyostelium特异性激酶中的许多可能与这种生物体应对饥饿的独特机制有关。
Dictyostelium discoideum is a widely studied model organism with both unicellular and multicellular forms in its developmental cycle. The Dictyostelium genome encodes 285 predicted protein kinases, similar to the count of the much more advanced Drosophila. It contains members of most kinase classes shared by fungi and metazoans, as well as many previously thought to be metazoan specific, indicating that they have been secondarily lost from the fungal lineage. This includes the entire tyrosine kinase–like (TKL) group, which is expanded in Dictyostelium and includes several novel receptor kinases. Dictyostelium lacks tyrosine kinase group kinases, and most tyrosine phosphorylation appears to be mediated by TKL kinases. About half of Dictyostelium kinases occur in subfamilies not present in yeast or metazoa, suggesting that protein kinases have played key roles in the adaptation of Dictyostelium to its habitat. This study offers insights into kinase evolution and provides a focus for signaling analysis in this system. Protein kinases are eukaryotic enzymes involved in cell communication pathways, and transmit information from outside the cell or between subcellular components within the cell. About 2.5% of genes code for protein kinases, and mutations in many of these cause human disease. The authors characterize the complete set of protein kinases (kinome) from Dictyostelium discoideum, a social amoeba that responds to starvation by forming aggregates of cells, which then differentiate into multicellular fruiting bodies. Dictyostelium branched from the vertebrate lineage after plants but before fungi, and thus illuminates an interesting period in evolutionary history. By comparing the Dictyostelium kinome to those of other organisms, the authors find 46 types of kinases that appear to be conserved in all organisms, and are likely to be involved in fundamental cellular processes. Dictyostelium is an established model organism for studying many aspects of cell biology that are conserved in humans, and this exposition of conserved kinases will help to guide future studies. The Dictyostelium kinome also contains an impressive degree of creativity—almost half of the kinases are unique to Dictyostelium. Many of these Dictyostelium-specific kinases may be related to this organism's distinctive mechanism for coping with starvation.
登录
查看更多内容
影响因子：
11.4
作者：
Bosgraaf, L;Russcher, H;Van Haastert, PJM
通讯作者：
Van Haastert, PJM
影响因子：
--
作者：
Chibalina, Margarita V;Anjard, Christophe;Insall, Robert H
通讯作者：
Insall, Robert H
影响因子：
--
作者：
Bimbó, A;Jia, YH;Liu, JH
通讯作者：
Liu, JH
DOI：
10.1073/pnas.0306880101
发表时间：
2004-08-10
影响因子：
11.1
作者：
Caenepeel, S;Charydczak, G;Manning, G
通讯作者：
Manning, G
影响因子：
3.3
作者：
Abysalh, JC;Kuchnicki, LL;Larochelle, DA
通讯作者：
Larochelle, DA