Untargeted Metabolic Profiling of Extracellular Vesicles of SARS-CoV-2-Infected Patients Shows Presence of Potent Anti-Inflammatory Metabolites.
Untargeted Metabolic Profiling of Extracellular Vesicles of SARS-CoV-2-Infected Patients Shows Presence of Potent Anti-Inflammatory Metabolites.
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DOI：
10.3390/ijms221910467
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发表时间：
2021-09-28
影响因子：
5.6
通讯作者：
Khan MI
中科院分区：
文献类型：
--
作者：
Alzahrani FA;Shait Mohammed MR;Alkarim S;Azhar EI;El-Magd MA;Hawsawi Y;Abdulaal WH;Yusuf A;Alhatmi A;Albiheyri R;Fakhurji B;Kurdi B;Madani TA;Alguridi H;Alosaimi RS;Khan MI
细胞外囊泡（EV）携带重要的生物分子，包括代谢产物，并有助于一些病毒的传播和致病。然而，到目前为止，关于EV代谢物含量的数据有限，这些代谢物可能在SARS-CoV-2病毒感染期间发挥关键作用。因此，本研究旨在进行非靶向代谢组学，以确定从COVID-19患者血清中分离的EV中存在的关键代谢物和相关途径。结果显示，在接受这些药物治疗的患者的EV中存在抗病毒药物和抗生素，如膦甲酸、茚地那韦和四环素。此外，与对照组相比，在COVID-19患者的EV中检测到抗炎代谢产物如LysoPS、7-α，25-二羟基胆固醇和15-d-PGJ 2水平升高。此外，我们发现COVID-19患者的EV中与凝血相关的代谢物（如血栓烷和反式脂肪酸）水平降低。这些发现表明，EV不仅携带活性药物分子，而且还携带抗炎代谢物，这清楚地表明外泌体可能在COVID-19感染期间与炎症加剧的谈判中发挥关键作用。这些初步结果也可能为鉴定新的代谢物铺平道路，这些代谢物可能在病毒感染期间充当炎症途径的关键调节剂。
Extracellular vesicles (EVs) carry important biomolecules, including metabolites, and contribute to the spread and pathogenesis of some viruses. However, to date, limited data are available on EV metabolite content that might play a crucial role during infection with the SARS-CoV-2 virus. Therefore, this study aimed to perform untargeted metabolomics to identify key metabolites and associated pathways that are present in EVs, isolated from the serum of COVID-19 patients. The results showed the presence of antivirals and antibiotics such as Foscarnet, Indinavir, and lymecycline in EVs from patients treated with these drugs. Moreover, increased levels of anti-inflammatory metabolites such as LysoPS, 7-α,25-Dihydroxycholesterol, and 15-d-PGJ2 were detected in EVs from COVID-19 patients when compared with controls. Further, we found decreased levels of metabolites associated with coagulation, such as thromboxane and elaidic acid, in EVs from COVID-19 patients. These findings suggest that EVs not only carry active drug molecules but also anti-inflammatory metabolites, clearly suggesting that exosomes might play a crucial role in negotiating with heightened inflammation during COVID-19 infection. These preliminary results could also pave the way for the identification of novel metabolites that might act as critical regulators of inflammatory pathways during viral infections.
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