Macrophages promote angiogenesis in human breast tumour spheroids in vivo.
Macrophages promote angiogenesis in human breast tumour spheroids in vivo.
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DOI：
10.1038/sj.bjc.6602901
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发表时间：
2006-01-16
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
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作者：
建立了一个体内模型，研究巨噬细胞在体内人乳腺肿瘤球体启动血管生成中的作用。将T47D球体植入裸鼠背部皮皱室，并与植入前人巨噬细胞渗入的球体诱导的血管生成反应进行了比较。我们的结果表明，在体外，与仅由肿瘤细胞组成的球体相比，巨噬细胞在球体中的存在导致血管内皮生长因子(VEGF)的释放至少增加三倍。在体内植入后3天，球体周围测量到的血管生成反应在被巨噬细胞渗透的球体中显著增强。血管数目增加(巨噬细胞与非巨噬细胞分别为34±1.9vs26±2.5，P<；0.008)，长度缩短(巨噬细胞与非巨噬细胞分别为116±4.92vs136±6.52vs136±6.52vsP<；0.008)，连接数目增加(巨噬细胞与非巨噬细胞分别为14±0.93vs11±1.25，P<；0.025)。这是第一个证明巨噬细胞在体内启动肿瘤血管生成中的作用的研究。
An in vivo model has been established to study the role of macrophages in the initiation of angiogenesis by human breast tumour spheroids in vivo. The extent of the angiogenic response induced by T47D spheroids implanted into the dorsal skinfold chamber in nude mice was measured in vivo and compared to that induced by spheroids infiltrated with human macrophages prior to implantation. Our results indicate that the presence of macrophages in spheroids resulted in at least a three-fold upregulation in the release of vascular endothelial growth factor (VEGF) in vitro when compared with spheroids composed only of tumour cells. The angiogenic response measured around the spheroids, 3 days after in vivo implantation, was significantly greater in the spheroids infiltrated with macrophages. The number of vessels increased (macrophages vs no macrophages 34±1.9 vs 26±2.5, P<0.01), were shorter in length (macrophages vs no macrophages 116±4.92 vs 136±6.52, P<0.008) with an increased number of junctions (macrophages vs no macrophages 14±0.93 vs 11±1.25, P<0.025) all parameters indicative of new vessel formation. This is the first study to demonstrate a role for macrophages in the initiation of tumour angiogenesis in vivo.
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影响因子：
8.8
作者：
通讯作者：
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影响因子：
8.8
作者：
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通讯作者：
McGee, J O
影响因子：
8.8
作者：
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通讯作者：
Coombes, R C
影响因子：
10.3
作者：
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通讯作者：
Dewhirst, MW
影响因子：
3.3
作者：
BUDZYNSKI, W;RADZIKOWSKI, C
通讯作者：
RADZIKOWSKI, C