Effect of vernalization on tuberization and flowering in the Tibetan turnip is associated with changes in the expression of FLC homologues.
Effect of vernalization on tuberization and flowering in the Tibetan turnip is associated with changes in the expression of FLC homologues.
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DOI：
10.1016/j.pld.2018.01.002
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发表时间：
2018-04
期刊：
影响因子：
4.8
通讯作者：
Yang Y
中科院分区：
文献类型：
--
作者：
Zheng Y;Luo L;Liu Y;Yang Y;Wang C;Kong X;Yang Y
芜菁（Brassica rapa var.芜菁）是一种两年生作物，在夏末/初秋种植，在温带地区形成肉质块茎供食用。收获的块茎然后越冬，并在第二年春天再次种植开花和种子。FLC是一种MADS盒转录因子，通过抑制开花启动子FT和SOC 1而作为花转变的主要阻遏物。在这里，我们表明，春化有效地抑制块茎的形成，促进西藏芜菁开花。我们的功能特性的四个FLC同源物（BrrFLC 1，FLC 2，FLC 3，和FLC 5），并发现BrrFLC 2和BrrFLC 1在抑制芜菁和转基因拟南芥开花中发挥重要作用。与此相反，块茎形成与BrrFLC 1在下胚轴中的表达相关，并且在BrrFLC 1的定量下调之后在冷处理下被抑制。非春化和春化芜菁嫁接实验表明，春化独立抑制块茎或下胚轴的砧木或接穗，这发生在平行的BrrFLC 1活性的减少块茎。总之，我们的研究结果表明，西藏萝卜是高度响应冷暴露，这是与BrrFLC基因的表达水平。
The turnip (Brassica rapa var. rapa) is a biennial crop that is planted in late summer/early fall and forms fleshy tubers for food in temperate regions. The harvested tubers then overwinter and are planted again the next spring for flowering and seeds. FLOWERING LOCUS C (FLC) is a MADS-box transcription factor that acts as a major repressor of floral transition by suppressing the flowering promoters FT and SOC1. Here we show that vernalization effectively represses tuber formation and promotes flowering in Tibetan turnip. We functionally characterized four FLC homologues (BrrFLC1, FLC2, FLC3, and FLC5), and found that BrrFLC2 and BrrFLC1 play a major role in repressing flowering in turnip and in transgenic Arabidopsis. In contrast, tuber formation was correlated with BrrFLC1 expression in the hypocotyl and was repressed under cold treatment following the quantitative downregulation of BrrFLC1. Grafting experiments of non-vernalized and vernalized turnips revealed that vernalization independently suppressed tuberization in the tuber or hypocotyl of the rootstock or scion, which occurred in parallel with the reduction in BrrFLC1 activity. Together, our results demonstrate that the Tibetan turnip is highly responsive to cold exposure, which is associated with the expression levels of BrrFLC genes.
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DOI：
10.1111/tpj.13221
发表时间：
2016-09
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Irwin JA;Soumpourou E;Lister C;Ligthart JD;Kennedy S;Dean C
通讯作者：
Dean C
影响因子：
7.4
作者：
Mendez-Vigo, Belen;Xavier Pico, F.;Alonso-Blanco, Carlos
通讯作者：
Alonso-Blanco, Carlos
影响因子：
7.4
作者：
Michaels, SD;Himelblau, E;Amasino, RM
通讯作者：
Amasino, RM
影响因子：
56.9
作者：
Yan, LL;Loukoianov, A;Dubcovsky, J
通讯作者：
Dubcovsky, J
影响因子：
7.4
作者：
Liang, Dacheng;White, Rosemary G.;Waterhouse, Peter M.
通讯作者：
Waterhouse, Peter M.