Changes in the interfacial shear resistance of disc annulus fibrosus from genipin crosslinking.
Changes in the interfacial shear resistance of disc annulus fibrosus from genipin crosslinking.
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DOI：
10.1016/j.jbiomech.2013.10.019
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发表时间：
2014-01-03
影响因子：
2.4
通讯作者：
Criscione, John
中科院分区：
文献类型：
--
作者：
Kirking, Bryan;Hedman, Thomas;Criscione, John
交联软组织在组织工程应用中已变得更加常见，并且最近的研究表明，软组织的机械行为可以通过交联直接改变。使用最近报道的剪切相邻椎间盘薄片的测试方法，使用体外牛椎间盘环研究京尼平交联对层间抗剪切性的影响。取新鲜冷冻牛尾标本3条，取4片，解剖相邻板层标本。将这些样本配对并在37°C下浸泡在具有20 mM京尼平的50 mM EPPS磷酸盐（ph 9）中4小时或浸泡在50 mM EPPS磷酸盐（ph 9）中，其中成功测试和分析了12个样本（每个处理6个）。与假处理对照相比，交联样本的屈服力/宽度（59%）、峰值力/宽度（70%）和弹性（69%）显著更高，支持京尼平交联增加瓣环界面层间剪切阻力的假设。此外，由于其他研究者先前描述的桥接纤维网络，层间剪切强度和相邻层之间可能存在依赖性。
Crosslinking soft tissue has become more common in tissue engineering applications, and recent studies have demonstrated that soft tissue mechanical behavior can be directly altered through crosslinking. Using a recently reported test method that shears adjacent disc lamella, the effect of genipin crosslinking on interlamellar shear resistance was studied using in vitro bovine disc annulus. Specimens of adjacent lamella were dissected from 4 discs taken from 3 fresh frozen bovine tails. These specimens were paired and soaked in either 50mM EPPS Phosphate (ph9) with 20mM genipin at 37°C for 4 hours or in 50mM EPPS Phosphate (ph9) of which twelve specimens (6 per treatment) were successfully tested and analyzed. Crosslinked specimens were noted to have significantly higher yield force per width (59%), peak force per width (70%), and resilience (69%) compared to sham treated controls, supporting the hypothesis that genipin crosslinking increases the resistance to interlamellar shear of the annulus interface. Additionally, a possible dependency may exist between the interlamellar shear strength and neighboring lamella because of the bridging fiber network previously described by other investigators.
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