Diverse expression of TNF-α and CCL27 in serum and blister of Stevens-Johnson syndrome/toxic epidermal necrolysis.
Diverse expression of TNF-α and CCL27 in serum and blister of Stevens-Johnson syndrome/toxic epidermal necrolysis.
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Stevens-Johnson综合征/中毒性表皮坏死松解症血清和水疱中TNF-α和CCL27的多样性表达
DOI：
10.1186/s13601-018-0199-6
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发表时间：
2018
影响因子：
4.4
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Wang F;Ye Y;Luo ZY;Gao Q;Luo DQ;Zhang X
Stevens-Johnson综合征（SJS）/中毒性表皮坏死松解症（TEN）的发病机制尚未完全了解。我们以前的研究报告趋化因子CCL 27在SJS/TEN患者血清中过表达。本研究的目的是研究急性期或消退期SJS/TEN患者血清和水疱液中的CCL 27和TNF-α水平。研究共招募了27名SJS/TEN患者和39名健康供体。采用酶联免疫吸附试验测定血清和囊泡中CCL 27和TNF-α的水平。SJS/TEN患者急性期血清CCL 27和TNF-α水平显著高于缓解期和正常对照组（P = 0.001/< 0.001; P = 0.012/< 0.001）。SJS/TEN患者缓解期血清TNF-α水平显著高于正常对照组（P < 0.001）。急性期血清CCL 27水平与TNF-α水平呈正相关（rs = 0.660; P < 0.001）。在急性期，水疱液中的CCL 27水平明显低于血清中的CCL 27水平（P = 0.008）。囊泡中TNF-α水平显著高于急性期血清（P = 0.040）和缓解期血清（P = 0.029）。本研究证实了CCL 27和TNF-α在SJS/TEN的发病过程中的作用。CCL 27可能通过循环在疾病过程的早期起作用，而TNF-α在整个疾病过程中在皮肤病变中起作用。
The pathogenesis of Stevens–Johnson syndrome (SJS)/toxic epidermal necrolysis (TEN) is not fully understood. Our previous study reported that chemokine CCL27 was overexpressed in serum of SJS/TEN patients. The objective of this study was to investigate the levels of CCL27 and TNF-α in serum and blister fluid from patients with SJS/TEN during the acute stage or resolution phase. A total of 27 patients with SJS/TEN and 39 healthy donors were recruited to the study. Serum and vesicular levels of CCL27 and TNF-α were determined by enzyme-linked immunosorbent assays. Serum levels of CCL27 and TNF-α were significantly elevated in patients with SJS/TEN during the acute stage as compared to the resolution phase and also compared with levels observed in normal controls (P = 0.001/< 0.001; P = 0.012/< 0.001). Serum TNF-α levels were significantly higher in patients with SJS/TEN during the resolution phase compared with normal controls (P < 0.001). Serum CCL27 levels were positively correlated with TNF-α levels during the acute stage (rs = 0.660; P < 0.001). Blister fluid exhibited much lower CCL27 levels than serum did during the acute stage (P = 0.008). TNF-α levels were much higher in vesicles in contrast to serum from acute stage (P = 0.040) as well as serum from resolution phase (P = 0.029). Our study demonstrated roles of CCL27 and TNF-α in promoting the course of SJS/TEN. CCL27 may act early in the course of disease, via the circulation, whereas TNF-α acts throughout the course of disease, in skin lesions.
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影响因子：
6.5
作者：
Viard-Leveugle, Isabelle;Gaide, Olivier;French, Lars E.
通讯作者：
French, Lars E.
影响因子：
4.1
作者：
van den Broek, Lenie J.;Kroeze, Kim L.;Gibbs, Susan
通讯作者：
Gibbs, Susan
影响因子：
5.9
作者：
Wang, Fang;He, Dingyang;Zhang, Xingqi
通讯作者：
Zhang, Xingqi
影响因子：
13.8
作者：
Paradisi, Andrea;Abeni, Damiano;Didona, Biagio
通讯作者：
Didona, Biagio
DOI：
10.1073/pnas.96.25.14470
发表时间：
1999-12-07
影响因子：
11.1
作者：
Morales, J;Homey, B;Zlotnik, A
通讯作者：
Zlotnik, A