RNA Taste Is Conserved in Dipteran Insects.
RNA Taste Is Conserved in Dipteran Insects.
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DOI：
10.1016/j.tjnut.2023.03.010
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发表时间：
2023-05
期刊：
影响因子：
--
通讯作者：
Amrein H
中科院分区：
文献类型：
--
作者：
Fujii S;Ahn JE;Jagge C;Shetty V;Janes C;Mohanty A;Slotman M;Adelman ZN;Amrein H
核糖核苷和RNA是果蝇幼虫发育和生长过程中不可或缺的营养物质。这些营养物质的检测需要由Gr 28基因编码的6种密切相关的味觉受体中的至少一种，Gr 28基因是最保守的昆虫味觉受体亚家族之一。我们调查了大约6500万年前和2.6亿年前分别与果蝇共享最后祖先的苍蝇幼虫和蚊子幼虫是否可以品尝RNA和核糖。我们还测试了埃及伊蚊和冈比亚按蚊的Gr 28同源基因在转基因果蝇幼虫中表达时是否能感受到这些营养物质。果蝇的味觉偏好进行了检查，采用2-选择偏好测定，已建立良好的果蝇幼虫。对于蚊子埃及伊蚊，我们开发了一种新的2-选择偏好测定，该测定适应这些昆虫幼虫的水生环境。最后，我们确定了这些物种中的Gr 28同源物，并在果蝇中表达，以确定其作为RNA受体的潜在功能。在二次选择性取食试验中，RNA（0.5mg/mL）对大花旋切蝇和铜绿绿蝇幼虫有强烈的引诱作用（P < 0.05）。类似地，在水生2-选择摄食测定中，埃及伊蚊幼虫显示出对RNA（2.5mg/mL）的强烈偏好。此外，当伊蚊或按蚊的Gr 28同源物在缺乏Gr 28基因的果蝇幼虫的食欲味觉神经元中表达时，对RNA（0.5 mg/mL）和核糖（0.1 M）的偏好被拯救（P < 0.05）。昆虫对RNA和核糖核苷的食欲出现在大约2.6亿年前，蚊子和果蝇从它们最后的共同祖先中分离出来。与糖受体一样，RNA受体在昆虫进化过程中高度保守，这表明RNA是快速生长的昆虫幼虫的关键营养素。
Ribonucleosides and RNA are an underappreciated nutrient group essential during Drosophila larval development and growth. Detection of these nutrients requires at least one of the 6 closely related taste receptors encoded by the Gr28 genes, one of the most conserved insect taste receptor subfamilies. We investigated whether blow fly larvae and mosquito larvae, which shared the last ancestor with Drosophila about 65 and 260 million years ago, respectively, can taste RNA and ribose. We also tested whether the Gr28 homologous genes of the mosquitoes Aedes aegypti and Anopheles gambiae can sense these nutrients when expressed in transgenic Drosophila larvae. Taste preference in blow flies was examined by adapting a 2-choice preference assay that has been well-established for Drosophila larvae. For the mosquito Aedes aegypti, we developed a new 2-choice preference assay that accommodates the aquatic environment of these insect larvae. Finally, we identified Gr28 homologs in these species and expressed them in Drosophila melanogaster to determine their potential function as RNA receptors. Larvae of the blow fly Cochliomyia macellaria and Lucilia cuprina are strongly attracted to RNA (0.5 mg/mL) in the 2-choice feeding assays (P < 0.05). Similarly, the mosquito Aedes aegypti larvae showed a strong preference for RNA (2.5 mg/mL) in an aquatic 2-choice feeding assay. Moreover, when Gr28 homologs of Aedes or Anopheles mosquitoes are expressed in appetitive taste neurons of Drosophila melanogaster larvae lacking their Gr28 genes, preference for RNA (0.5 mg/mL) and ribose (0.1 M) is rescued (P < 0.05). The appetitive taste for RNA and ribonucleosides in insects emerged about 260 million years ago, the time mosquitoes and fruit flies diverged from their last common ancestor. Like sugar receptors, receptors for RNA have been highly conserved during insect evolution, suggesting that RNA is a critical nutrient for fast-growing insect larvae.
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影响因子：
64.5
作者：
Miyamoto T;Slone J;Song X;Amrein H
通讯作者：
Amrein H
DOI：
10.1016/j.tig.2015.09.005
发表时间：
2015-12
期刊：
Trends in genetics : TIG
影响因子：
--
作者：
Joseph RM;Carlson JR
通讯作者：
Carlson JR
影响因子：
2.2
作者：
BARBER, JT;ELLGAARD, EG;HERSKOWITZ, K
通讯作者：
HERSKOWITZ, K
影响因子：
2.2
作者：
DADD, RH
通讯作者：
DADD, RH
影响因子：
3
作者：
Engsontia P;Satasook C
通讯作者：
Satasook C