Six weeks of voluntary wheel running modulates inflammatory protein (MCP-1, IL-6, and IL-10) and DAMP (Hsp72) responses to acute stress in white adipose tissue of lean rats.
Six weeks of voluntary wheel running modulates inflammatory protein (MCP-1, IL-6, and IL-10) and DAMP (Hsp72) responses to acute stress in white adipose tissue of lean rats.
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DOI：
10.1016/j.bbi.2013.10.028
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发表时间：
2014-07
影响因子：
15.1
通讯作者：
Fleshner, Monika
中科院分区：
文献类型：
--
作者：
Speaker, Kristin J.;Cox, Stewart S.;Paton, Madeline M.;Serebrakian, Arman;Maslanik, Thomas;Greenwood, Benjamin N.;Fleshner, Monika
关键词：
为了使局部组织准备好应对潜在的感染或损伤，暴露在急性、强烈的应激源下会引起循环和局部组织炎症蛋白的增加。有规律的体力活动有助于应激诱发的先天反应性，并调节免疫代谢组织如白色脂肪组织(WAT)中炎性蛋白的表达。然而，有规律的体力活动对WAT中应激诱发的炎性蛋白表达的影响尚不清楚。为了研究这个问题，F344瘦雄性大鼠(体重150-175g)被允许自愿进入跑轮6周，然后暴露于急性应激源(100，1.5 mA-5 S不可避免的尾部电击)。用ELISA、皮质酮、热休克蛋白72、巨噬细胞趋化蛋白-1、肿瘤坏死因子-α、白介素1β、IL-6和IL-10测定血浆和皮下、腹膜(附属膜和腹膜后水池)和内脏(大网膜和肠系膜水池)水箱中的IL-6和IL-10浓度。急性应激增加了除肿瘤坏死因子-α外的所有蛋白质的血浆浓度，并根据检查的间隔，增加了单核细胞趋化蛋白-1、IL-1β、IL-6和IL-10的浓度。运动普遍增加WAT内IL-1β，增强应激诱导的血浆和WAT中的HSP72，并不同程度地增加WAT内应激诱导的单核细胞趋化蛋白-1、IL-6和IL-10。这些数据表明：(A)非肥胖WAT中的炎性蛋白可能在急性应激反应中发挥特定的免疫和代谢作用；(B)自愿习惯性运动可能通过增加应激诱导的HSP72、单核细胞趋化蛋白-1、IL-6和IL-10以及白色脂肪组织中IL-1β/IL 10和肿瘤坏死因子-α/IL 10的比率来优化应激诱导的天然免疫功能的增强。
To prime local tissues for dealing with potential infection or injury, exposure to an acute, intense stressor evokes increases in circulating and local tissue inflammatory proteins. Regular physical activity facilitates stress-evoked innate reactivity and modulates the expression of inflammatory proteins in immuno-metabolic tissues such as white adipose tissue (WAT). The impact of regular physical activity on stress-evoked inflammatory protein expression in WAT, however, remains unclear. To investigate this question, lean male F344 rats (150–175 g) were allowed voluntary access to a running wheel for 6 weeks followed by exposure to an acute stressor (100, 1.5 mA-5 s inescapable tail shocks). Using ELISAs, corticosterone, heat shock protein 72 (Hsp72), macrophage chemoattractant protein (MCP-1), tumor necrosis factor-alpha (TNF-α), interleukin (IL)-1β, IL-6, and IL-10 concentrations were measured in plasma and subcutaneous, intraperitoneal (epididymal and retroperitoneal WAT depots) and visceral (omental and mesenteric WAT depots) WAT compartments. Acute stress increased plasma concentrations of all proteins except TNF-α and, depending upon the compartment examined, WAT concentrations of MCP-1, IL-1β, IL-6, and IL-10. Exercise ubiquitously increased IL-1β within WAT, potentiated stress-evoked Hsp72 in plasma and WAT, and differentially increased stress-evoked MCP-1, IL-6, and IL-10 within WAT. These data suggest: (a) inflammatory proteins in non-obese WAT may serve compartment-specific immune and metabolic roles important to the acute stress response and; (b) voluntary habitual exercise may optimize stress-induced augmentation of innate immune function through increases in stress-evoked Hsp72, MCP-1, IL-6, and IL-10 and decreases in IL-1β/IL10 and TNF-α/IL10 ratios within white adipose tissue.
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DOI：
10.3109/10253890.2011.596865
发表时间：
2011-09
期刊：
Stress (Amsterdam, Netherlands)
影响因子：
--
作者：
Fleshner M;Maier SF;Lyons DM;Raskind MA
通讯作者：
Raskind MA
DOI：
10.1152/ajpregu.00513.2002
发表时间：
2003-02-01
影响因子：
2.8
作者：
Campisi, J;Leem, TH;Fleshner, M
通讯作者：
Fleshner, M
影响因子：
3.3
作者：
Campisi, J;Fleshner, M
通讯作者：
Fleshner, M
DOI：
10.3109/10253890.2011.653596
发表时间：
2012-11-01
影响因子：
2.3
作者：
Campisi, Jay;Sharkey, Craig;Fleshner, Monika
通讯作者：
Fleshner, Monika
影响因子：
3.2
作者：
Campeau S;Nyhuis TJ;Sasse SK;Kryskow EM;Herlihy L;Masini CV;Babb JA;Greenwood BN;Fleshner M;Day HE
通讯作者：
Day HE