Transcription shapes genome-wide histone acetylation patterns.
Transcription shapes genome-wide histone acetylation patterns.
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DOI：
10.1038/s41467-020-20543-z
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发表时间：
2021-01-11
影响因子：
16.6
通讯作者：
Howe LJ
中科院分区：
文献类型：
--
作者：
Martin BJE;Brind'Amour J;Kuzmin A;Jensen KN;Liu ZC;Lorincz M;Howe LJ
组蛋白乙酰化是一个普遍存在的转录标志，但组蛋白乙酰化与转录之间的联系是因果关系还是间接关系尚未得到解决。利用酿酒酵母的免疫印迹和染色质免疫沉淀测序，我们发现大部分组蛋白乙酰化依赖于转录。这种依赖性部分解释为RNA聚合酶II （RNAPII）需要H4组蛋白乙酰转移酶（HATs）与基因体相互作用。我们的数据也证实了转录激活子对HATs的靶向作用，但有趣的是，启动子结合的HATs在没有转录的情况下无法使组蛋白乙酰化。事实上，仅凭HAT占据率很难预测全基因组组蛋白乙酰化，这表明HAT活性在招募后受到调节。与此一致的是，我们发现组蛋白乙酰化在核小体上增加，预计会阻止RNAPII，支持这种修饰依赖于转录过程中核小体破坏的假设。总的来说，这些数据表明，组蛋白乙酰化是RNAPII促进组蛋白乙酰转移酶的募集和活性的结果。组蛋白乙酰化是一个普遍存在的转录标志。在这里，作者提供的证据表明，大部分组蛋白乙酰化依赖于转录，特别是由于RNAPII对组蛋白乙酰转移酶的募集和活性的要求。
Histone acetylation is a ubiquitous hallmark of transcription, but whether the link between histone acetylation and transcription is causal or consequential has not been addressed. Using immunoblot and chromatin immunoprecipitation-sequencing in S. cerevisiae, here we show that the majority of histone acetylation is dependent on transcription. This dependency is partially explained by the requirement of RNA polymerase II (RNAPII) for the interaction of H4 histone acetyltransferases (HATs) with gene bodies. Our data also confirms the targeting of HATs by transcription activators, but interestingly, promoter-bound HATs are unable to acetylate histones in the absence of transcription. Indeed, HAT occupancy alone poorly predicts histone acetylation genome-wide, suggesting that HAT activity is regulated post-recruitment. Consistent with this, we show that histone acetylation increases at nucleosomes predicted to stall RNAPII, supporting the hypothesis that this modification is dependent on nucleosome disruption during transcription. Collectively, these data show that histone acetylation is a consequence of RNAPII promoting both the recruitment and activity of histone acetyltransferases. Histone acetylation is a ubiquitous hallmark of transcription. Here the authors provide evidence that the majority of histone acetylation is dependent on transcription, specifically due to the requirement of RNAPII for the recruitment and activity of histone acetyltransferases.
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影响因子：
16.6
作者：
Gatchalian J;Wang X;Ikebe J;Cox KL;Tencer AH;Zhang Y;Burge NL;Di L;Gibson MD;Musselman CA;Poirier MG;Kono H;Hayes JJ;Kutateladze TG
通讯作者：
Kutateladze TG
影响因子：
16.8
作者：
Chen PB;Chen HV;Acharya D;Rando OJ;Fazzio TG
通讯作者：
Fazzio TG
影响因子：
64.5
作者：
Bose DA;Donahue G;Reinberg D;Shiekhattar R;Bonasio R;Berger SL
通讯作者：
Berger SL
影响因子：
4
作者：
Downey M;Baetz K
通讯作者：
Baetz K
影响因子：
16.6
作者：
Farnung, Lucas;Vos, Seychelle M.;Cramer, Patrick
通讯作者：
Cramer, Patrick