Overcoming Major Barriers to Developing Successful Sensors for Practical Applications Using Functional Nucleic Acids.
Overcoming Major Barriers to Developing Successful Sensors for Practical Applications Using Functional Nucleic Acids.
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DOI：
10.1146/annurev-anchem-061020-104216
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发表时间：
2022-06-13
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影响因子：
--
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多年来，人们一直致力于开发用于环境监测、食品安全和医疗诊断应用的灵敏、选择性和实用的传感器。然而，从创新研究到商业成功的转变相对较少。在这篇综述中，我们确定了几个障碍，开发成功的传感器的实际应用，包括缺乏通用的方法来（a）产生受体的广泛的目标，（B）提高传感器的选择性，以克服干扰，（c）选择性结合不同的光学，电化学，和其他信号，（d）调整动态范围，以匹配不同的目标所需的检测阈值。然后，我们总结了解决方案，以克服这些障碍，使用传感器的基础上功能核酸，包括DNA酶，适体和适体酶，以及如何将这些传感器耦合到广泛使用的测量设备，以扩大其功能，降低其在现场和护理点设置的实际应用的障碍。
For many years, numerous efforts have been focused on the development of sensitive, selective, and practical sensors for environmental monitoring, food safety, and medical diagnostic applications. However, the transition from innovative research to commercial success is relatively sparse. In this review, we identify several barriers to developing successful sensors for practical applications, including the lack of general methods to (a) generate receptors for a wide range of targets, (b) improve sensor selectivity to overcome interferences, (c) transduce the selective binding to different optical, electrochemical, and other signals, and (d) tune dynamic range to match thresholds of detection required for different targets. We then summarize solutions to overcome these barriers using sensors based on functional nucleic acids that include DNAzymes, aptamers, and aptazymes and how these sensors are coupled to widely available measurement devices to expand their capabilities and lower the barrier for their practical applications in the field and point-of-care settings.
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影响因子：
16.6
作者：
Hwang, Kevin;Wu, Peiwen;Kim, Taejin;Lei, Lei;Tian, Shiliang;Wang, Yingxiao;Lu, Yi
通讯作者：
Lu, Yi
DOI：
10.1007/978-1-61779-545-9_18
发表时间：
2012-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Ihms, Hannah E;Lu, Yi
通讯作者：
Lu, Yi
影响因子：
15
作者：
Guo, Zhong;Murphy, Lindy;Alexandrov, Kirill
通讯作者：
Alexandrov, Kirill
影响因子：
15
作者：
Chen, Ting-Ting;Tian, Xue;Li, Yingfu
通讯作者：
Li, Yingfu
影响因子：
16.6
作者：
Kang, Di;Vallee-Belisle, Alexis;Porchetta, Alessandro;Plaxco, Kevin W.;Ricci, Francesco
通讯作者：
Ricci, Francesco