Re-engineering electrochemical biosensors to narrow or extend their useful dynamic range.

Re-engineering electrochemical biosensors to narrow or extend their useful dynamic range.
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DOI:
10.1002/anie.201202204
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发表时间:
2012-07-02
影响因子:
16.6
通讯作者:
Ricci, Francesco
Ricci, Francesco
中科院分区:
化学1区
文献类型:
--
作者:
Kang, Di;Vallee-Belisle, Alexis;Porchetta, Alessandro;Plaxco, Kevin W.;Ricci, Francesco

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在这里,我们展示了两种方便的方法来扩展和缩小模型电化学DNA传感器的有用动态范围。我们通过在同一电极上结合具有不同靶亲和力但具有相似特异性的DNA探针来实现。我们能够在目标浓度中实现跨越3个数量级的扩展动态响应。使用不同的策略,我们还将E-DNA传感器的有用动态范围缩小到目标浓度的8倍范围。
Here we demonstrate two convenient methods to extend and narrow the useful dynamic range of a model electrochemical DNA sensor. We did so by combining DNA probes of different target affinities but with similar specificity on the same electrode. We were able to achieve an extended dynamic response spanning 3 orders of magnitude in target concentration. Using a different strategy we have also narrowed the useful dynamic range of an E-DNA sensor to only an 8-fold range of target concentrations.
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