Integrated device for optical stimulation and spatiotemporal electrical recording of neural activity in light-sensitized brain tissue.
Integrated device for optical stimulation and spatiotemporal electrical recording of neural activity in light-sensitized brain tissue.
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DOI：
10.1088/1741-2560/6/5/055007
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发表时间：
2009-10
影响因子：
4
通讯作者：
Nurmikko AV
中科院分区：
文献类型：
--
作者：
Zhang J;Laiwalla F;Kim JA;Urabe H;Van Wagenen R;Song YK;Connors BW;Zhang F;Deisseroth K;Nurmikko AV
具有高空间和时间精度的神经刺激对于研究神经网络的实时动力学和神经疾病的前瞻性临床治疗都是期望的。光学刺激的基因靶向神经元表达的光敏通道蛋白视紫红质（ChR2）最近已被报道为一种手段，毫秒的时间控制神经元尖峰活动与细胞类型的选择性。这提供了能够在刺激部位附近和远离刺激部位的地方局部递送光学刺激和通过电生理学手段同时监测神经活动的前景。我们在这里报告了一种新型的双模态混合装置，它包括一个锥形同轴光波导（“optrode”）集成到一个100个元件的皮层内多电极记录阵列。我们首先证明了双光学传递和电记录能力的单optrode在体外制备的小鼠视网膜，光刺激的天然视网膜光感受器，同时记录光响应活动的神经节细胞。然后在ChR2转染的小鼠脑切片中使用双模态阵列装置。具体而言，癫痫样事件是可靠的光学触发的光电极和他们的时空模式同时记录的多电极阵列。
Neural stimulation with high spatial and temporal precision is desirable both for studying the real-time dynamics of neural networks and for prospective clinical treatment of neurological diseases. Optical stimulation of genetically targeted neurons expressing the light sensitive channel protein Channelrhodopsin (ChR2) has recently been reported as a means for millisecond temporal control of neuronal spiking activities with cell-type selectivity. This offers the prospect of enabling local delivery of optical stimulation and the simultaneous monitoring of the neural activity by electrophysiological means, both in the vicinity of and distant to the stimulation site. We report here a novel dual-modality hybrid device, which consists of a tapered coaxial optical waveguide (‘optrode’) integrated into a 100 element intra-cortical multi-electrode recording array. We first demonstrate the dual optical delivery and electrical recording capability of the single optrode in in vitro preparations of mouse retina, photo-stimulating the native retinal photoreceptors while recording light-responsive activities from ganglion cells. The dual-modality array device was then used in ChR2 transfected mouse brain slices. Specifically, epileptiform events were reliably optically triggered by the optrode and their spatiotemporal patterns were simultaneously recorded by the multi-electrode array.
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影响因子：
25
作者：
Cruikshank, Scott J.;Lewis, Timothy J.;Connors, Barry W.
通讯作者：
Connors, Barry W.
影响因子：
16.2
作者：
Elstrott, Justin;Anishchenko, Anastasia;Feller, Marla B.
通讯作者：
Feller, Marla B.
影响因子：
25
作者：
Boyden, ES;Zhang, F;Deisseroth, K
通讯作者：
Deisseroth, K
影响因子：
64.8
作者：
Adamantidis, Antoine R.;Zhang, Feng;De Lecea, Luis
通讯作者：
De Lecea, Luis
影响因子：
4
作者：
Aravanis, Alexander M.;Wang, Li-Ping;Deisseroth, Karl
通讯作者：
Deisseroth, Karl