A comparative study on the regulatory region of the PERIOD1 gene among diurnal/nocturnal primates.
A comparative study on the regulatory region of the PERIOD1 gene among diurnal/nocturnal primates.
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DOI：
10.1186/s40101-016-0111-9
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发表时间：
2016-09-28
影响因子：
3.1
通讯作者：
Oota H
中科院分区：
文献类型：
--
作者：
Katsumura T;Fukuyo Y;Kawamura S;Oota H
昼夜节律伴随着生理和新陈代谢节律，在白天醒着、晚上睡着的人类体内，生物钟设定在24小时左右。大多数单目灵长类动物，包括人类，都是白天活动的，而大多数“原始的”链状灵长类灵长类动物是夜间活动的，这表明灵长类动物已经从夜间活动进化到了白天的习性。然而，导致习惯的生理变化和导致生理变化的基因变化的机制尚不清楚。为了揭示这些机制，我们专注于PERIOD1(PER1)基因调节区的核苷酸序列，该基因被认为是哺乳动物生物钟的关键元件之一。我们测定了6种灵长类动物PER1基因表达调控区的核苷酸序列，并与国际DNA数据库中8种灵长类动物的序列进行了比较。基于序列数据，我们构建了包含昼夜活动物种的系统发育树，并研究了该调节区的鸟嘌呤和胞嘧啶(GC)含量。在所研究的灵长类动物中，调控基因表达的基序在进化上是保守的。系统发育树仅显示了物种间的系统发育关系，没有区分昼夜类群的分枝模式。我们发现，与PER1调节区的GC含量相关的两个核心在白天和夜间的习性之间显示出统计上的显着差异。我们的结果提示，PER1上游区域的两个核心可能与导致昼夜活动灵长类动物行为差异的基因表达调控有关。本文的在线版本(doi：10.1186/s40101-0160111-9)包含补充材料，授权用户可以使用。
The circadian clock is set up around a 24-h period in humans who are awake in the daytime and sleep in the nighttime, accompanied with physiological and metabolic rhythms. Most haplorhine primates, including humans, are diurnal, while most “primitive” strepsirrhine primates are nocturnal, suggesting primates have evolved from nocturnal to diurnal habits. The mechanisms of physiological changes causing the habits and of genetic changes causing the physiological changes are, however, unknown. To reveal these mechanisms, we focus on the nucleotide sequences of the regulatory region of the PERIOD1 (PER1) gene that is known as one of the key elements of the circadian clock in mammalians. We determined nucleotide sequences of the regulatory region of PER1 concerning the gene expression for six primates and compared those with those of eight primates from the international DNA database. Based on the sequence data, we constructed a phylogenetic tree including both the diurnal/nocturnal species and investigated the guanine and cytosine (GC) content in the regulatory region. The motif sequences regulating gene expression were evolutionary conservative in the primates examined. The phylogenetic tree simply showed phylogenetic relationship among the species and no branching pattern distinguishable between the diurnal and nocturnal groups. We found two cores showing a statistically significant difference between the diurnal and the nocturnal habits related to the GC contents of the regulatory region of PER1. Our results suggest the possibility that the two cores in the upstream region of PER1 are related to the regulation of gene expression leading to behavioral differences between diurnal and nocturnal primates. The online version of this article (doi:10.1186/s40101-016-0111-9) contains supplementary material, which is available to authorized users.
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DOI：
10.1186/1297-9686-45-9
发表时间：
2013-04-04
期刊：
Genetics, selection, evolution : GSE
影响因子：
--
作者：
Rao YS;Chai XW;Wang ZF;Nie QH;Zhang XQ
通讯作者：
Zhang XQ
影响因子：
14.9
作者：
Vinogradov, AE
通讯作者：
Vinogradov, AE
DOI：
10.1093/bioinformatics/btp348
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Capella-Gutiérrez S;Silla-Martínez JM;Gabaldón T
通讯作者：
Gabaldón T
影响因子：
3.9
作者：
HASEGAWA, M;KISHINO, H;YANO, TA
通讯作者：
YANO, TA
影响因子：
3.5
作者：
Sémon, M;Mouchiroud, D;Duret, L
通讯作者：
Duret, L