Protective Effects of Genistein against Mono-(2-ethylhexyl) Phthalate-Induced Oxidative Damage in Prepubertal Sertoli Cells.
Protective Effects of Genistein against Mono-(2-ethylhexyl) Phthalate-Induced Oxidative Damage in Prepubertal Sertoli Cells.
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金雀异黄素对青春期前支持细胞中邻苯二甲酸单（2-乙基己基）诱导的氧化损伤的保护作用。
DOI：
10.1155/2017/2032697
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发表时间：
2017
影响因子：
--
通讯作者：
Li H
中科院分区：
文献类型：
--
作者：
Zhang L;Gao M;Zhang T;Chong T;Wang Z;Zhai X;Wu Z;Li H
邻苯二甲酸单-（2-乙基己基）酯（MEHP）和染料木素是环境和食物中最常见的两种内分泌干扰化学物质（EDCs）。然而，这两种EDCs如何影响青春期前支持细胞的发育却鲜有研究。本研究分离22日龄Sprague Dawley大鼠的青春期前支持细胞，分别暴露于1 μmol/L、10 μmol/L和100 μmol/L （M1、M10和M100）的MEHP和10 μmol/L (G)的染料木素及其组合（G + M1、G + M10和G + M100）。观察细胞增殖抑制率、凋亡坏死率和细胞氧化还原状态。结果表明，MEHP能显著提高细胞增殖抑制率、凋亡率、坏死率和细胞内活性氧化物质水平。然而，染料木素可通过增强mehp诱导的睾丸抗氧化酶活性和上调Nrf2和HO-1表达，部分减轻mehp诱导的青春期前支持细胞氧化损伤，表明染料木素可通过抗氧化作用部分减轻mehp诱导的青春期前支持细胞损伤，在治疗其他edcs诱导的生殖疾病方面具有广阔的应用前景。
Mono-(2-ethylhexyl) phthalate (MEHP) and genistein are two of the most prevalent endocrine-disrupting chemicals (EDCs) that present in the environment and food. However, how these two EDCs would affect prepubertal Sertoli cells development was rarely studied. In this study, primary prepubertal Sertoli cells were isolated from 22-day-old Sprague Dawley rats and exposed to MEHP at 1 μmol/L, 10 μmol/L, and 100 μmol/L (M1, M10, and M100), genistein at 10 μmol/L (G), and their combination (G + M1, G + M10, and G + M100). Cell proliferation inhibition rate, apoptosis and necrosis rate, and cellular redox state were evaluated. Our results revealed that MEHP could significantly increase cell proliferation inhibition rate, apoptosis rate, necrosis rate, and intracellular reactive oxidative species level. However, coadministration of genistein could partially alleviate MEHP-induced prepubertal Sertoli cells oxidative injuries via enhancement of testicular antioxidative enzymes activities and upregulation of Nrf2 and HO-1, indicating that genistein could partially attenuate MEHP-induced prepubertal Sertoli cells damage through antioxidative action and may have promising future on its curative role for attenuating other EDCs-induced reproductive disorders.
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影响因子：
4.2
作者：
Qian, Yisong;Guan, Teng;Yu, Deyue
通讯作者：
Yu, Deyue
影响因子：
9.8
作者：
Kim, Sunmi;Kang, Sungeun;Choi, Kyungho
通讯作者：
Choi, Kyungho
影响因子：
4.1
作者：
Kasahara, E;Sato, EF;Inoue, M
通讯作者：
Inoue, M
影响因子：
3.6
作者：
Maines, MD;Ewing, JF
通讯作者：
Ewing, JF
DOI：
10.1038/jes.2008.44
发表时间：
2009-02
影响因子：
4.5
作者：
Cao, Yang;Calafat, Antonia M.;Doerge, Daniel R.;Umbach, David M.;Bernbaum, Judy C.;Twaddle, Nathan C.;Ye, Xiaoyun;Rogan, Walter J.
通讯作者：
Rogan, Walter J.