Isoflavones in urine, saliva, and blood of infants: data from a pilot study on the estrogenic activity of soy formula.
Isoflavones in urine, saliva, and blood of infants: data from a pilot study on the estrogenic activity of soy formula.
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DOI：
10.1038/jes.2008.44
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发表时间：
2009-02
影响因子：
4.5
通讯作者：
Rogan, Walter J.
中科院分区：
文献类型：
--
作者：
Cao, Yang;Calafat, Antonia M.;Doerge, Daniel R.;Umbach, David M.;Bernbaum, Judy C.;Twaddle, Nathan C.;Ye, Xiaoyun;Rogan, Walter J.
在美国，约25%的在售婴儿配方奶粉以大豆蛋白为基础，大豆蛋白是人类食物供应中雌激素类异黄酮的重要来源。然而，很少有研究报告婴儿体内异黄酮的水平。我们进行了一项部分横断面、部分纵向的试点研究，以检测儿童因不同喂养方式而接触到的异黄酮情况。根据喂养史，招募了166名出生至1岁的足月婴儿，分别采用大豆配方奶粉、牛奶配方奶粉或母乳喂养。在382次随访中，收集了381份尿液、361份唾液和88份血液样本。我们使用自动化在线固相萃取结合高效液相色谱 - 串联质谱法来测量尿液中的三种异黄酮（大豆苷元、染料木黄酮和雌马酚），并对唾液和血斑使用类似的液相色谱/串联质谱技术。在大多数母乳喂养或牛奶配方奶粉喂养的儿童的血液或唾液样本中，检测不到大豆苷元和染料木黄酮的浓度。尿液中未检测到的比例比其他基质略低。仅在少数尿液样本中可检测到雌马酚的浓度。对于大豆苷元和染料木黄酮，尿液中的中位浓度最高，其次是血液，然后是唾液。食用大豆配方奶粉的婴儿尿液中染料木黄酮和大豆苷元的浓度比食用牛奶配方奶粉的婴儿高约500倍。任何一种分析物在不同基质之间的相关性都显著低于在单一基质中两种分析物之间的相关性。我们没有发现食用大豆配方奶粉的儿童体内异黄酮浓度与某些激素水平之间存在显著相关性。我们的研究结果基于数量多得多的婴儿，有力地证实了先前的报告，但大豆配方奶粉中的植物雌激素在婴儿体内是否具有生物活性仍然是一个悬而未决的问题。我们计划进一步开展纵向研究，重点关注反映雌激素暴露影响的身体和发育方面的结果。
In the United States, about 25% of infant formula sold is based on soy protein, which is an important source of estrogenic isoflavones in the human food supply. Nevertheless, few studies report isoflavones levels in infants. We did a partly cross-sectional, partly longitudinal pilot study to examine children’s exposure to isoflavones from different feeding methods. One hundred sixty-six full term infants between birth and 1 year of age were recruited into soy formula, cow milk formula or breast milk regimens according to their feeding histories. Three hundred eighty-one urine, 361 saliva and 88 blood samples were collected at 382 visits. We used automated online SPE coupled to HPLC-MS/MS for measuring three isoflavones (daidzein, genistein, and equol) in urine, and use similar LC/MS/MS techniques for saliva and blood spots . Concentrations of daidzein and genistein were undetectable in most blood or saliva samples from children fed breast milk or cow-milk formula. The proportion of non-detectable values was somewhat lower in urine than the other matrices. Concentrations of equol were detectable in only a few urine samples. For both daidzein and genistein, urine contained the highest median concentrations, followed by blood, and then saliva. Urinary concentrations of genistein and daidzein were about 500 times higher in the soy-formula-fed infants than in the cow-milk-formula-fed infants. The correlations between matrices for either analyte were strikingly lower than the correlation between the two analytes in any single matrix. We did not find significant correlations between isoflavone concentrations and the levels of certain hormones in children fed soy formula. Our results, based on much larger numbers of infants, strongly confirm previous reports, but whether the phytoestrogens in soy formula are biologically active in infants is still an open question. We plan further longitudinal studies focusing on physical and developmental findings reflecting the effects of estrogen exposure.
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影响因子：
10.4
作者：
Lubin JH;Colt JS;Camann D;Davis S;Cerhan JR;Severson RK;Bernstein L;Hartge P
通讯作者：
Hartge P
影响因子：
2
作者：
COOK, DA
通讯作者：
COOK, DA
影响因子：
2.9
作者：
Franke, AA;Custer, LJ;Hundahl, SA
通讯作者：
Hundahl, SA
影响因子：
4.2
作者：
ANDERSON, RL;WOLF, WJ
通讯作者：
WOLF, WJ
影响因子：
4.5
作者：
MAYR, U;BUTSCH, A;SCHNEIDER, S
通讯作者：
SCHNEIDER, S