Female hormone receptors are differentially expressed in mouse fibrocartilages.
Female hormone receptors are differentially expressed in mouse fibrocartilages.
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DOI：
10.1016/j.joca.2008.09.015
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发表时间：
2009-05
影响因子：
7
通讯作者：
Kapila S
中科院分区：
文献类型：
--
作者：
Wang W;Hayami T;Kapila S
尽管女性易患关节疾病，而且已知女性激素在调节软骨细胞功能中的作用，但关节中的各种女性激素受体亚型还没有得到很好的描述，关节和性别之间的受体谱也缺乏比较。本研究比较了雌激素受体(ER)-α和-β、松弛素受体LGR7和LGR8以及孕激素受体(PR)在颞下颌关节盘、膝关节半月板和耻骨联合纤维软骨中的相对水平。12周龄小鼠的纤维软骨细胞在含α-MEM的血清中维持，直到融合。采用RT-PCR法、qRT-PCR法、免疫细胞化学法和免疫印迹法检测总RNA和细胞裂解产物，关节切片进行免疫组织化学检测。所有被检测的激素受体都存在于三个关节中，但在不同关节之间的表达水平存在显著差异。TMJ细胞的ER-α(>；2.8倍)、ER-β(>；2.2倍)、LGR7(>；3倍)和PR(>；1.8倍)高于膝关节半月板细胞，而LGR8(0.5倍)基因表达水平低于膝关节半月板细胞。TMJ中ER-α/ER-β和LGR7/LGR8的比值分别是半月板细胞的1.8%和7.5%。TMJ盘内激素受体分布与耻骨联合相似。免疫组织化学证实了这些受体在三种组织中的差异表达模式。TMJ细胞在PR水平上表现出性别二型性。这一发现表明，这些纤维软骨可能是女性激素作用的靶组织。激素受体在三种关节纤维软骨中的差异表达谱和TMJ间盘细胞中ER的性别二型性可能导致这些纤维软骨组织内激素反应的不同下游效应。
Despite the female predilection for joint diseases, and the known effects of female hormones in regulating chondrocyte function, the various female hormone receptor subtypes in joints are not well characterized, and comparisons in receptor profiles between joints and genders is lacking. This investigation characterized and compared the relative levels of estrogen receptors (ER) –α and -β, relaxin receptors LGR7 and LGR8, and progesterone receptor (PR) in the temporomandibular joint (TMJ) disc, knee meniscus and pubic symphysis fibrocartilages. Fibrocartilaginous cells from 12-week old mice were maintained in serum-containing α-MEM until confluence. Total RNA and cell lysates were assayed by RT-PCR, qRT-PCR, immunocytochemistry and Western blots, and joint sections subjected to immunohistochemistry. All hormone receptors assayed were present in the three joints, but showed substantial differences in expression levels between joints. TMJ cells had higher ER-α (>2.8-fold), ER-β (>2.2-fold), LGR7 (>3-fold) and PR (>1.8-fold), and lower LGR8 (0.5-fold) gene expression levels than knee meniscus cells. The ratio of ER-α:ER-β and LGR7:LGR8 was 1.8- and 7.5-fold higher, respectively, in TMJ than in knee meniscus cells. The profile of hormone receptors in the TMJ disc were similar to those in the pubic symphysis. Immunochemistry confirmed the differential expression patterns of these receptors in the three tissues. The TMJ cells demonstrated sexual dimorphism in the levels of PR. The findings suggest that these fibrocartilages are putative target tissues for actions of female hormones. The differential expression profiles of the hormone receptors in the three joint fibrocartilages and the sexual dimorphism in ERs in TMJ disc cells are likely to result in varied downstream effects in response to hormones within these fibrocartilaginous tissues.
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影响因子：
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通讯作者：
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