Apterous A modulates wing size, bristle formation and patterning in Nilaparvata lugens.
Apterous A modulates wing size, bristle formation and patterning in Nilaparvata lugens.
复制标题
Apterous A 调节褐飞虱的翅膀大小、刚毛形成和图案
DOI：
10.1038/srep10526
复制
发表时间：
2015-05-21
影响因子：
4.6
通讯作者：
Hua H
中科院分区：
文献类型：
--
作者：
Liu F;Li K;Li J;Hu D;Zhao J;He Y;Zou Y;Feng Y;Hua H
Apterous A （apA）是LIM-homeobox基因家族的成员，在翅膀的发育中起着至关重要的作用。刚毛-鳞片复合体（AS-C）编码基本螺旋-环-螺旋（bHLH）转录因子，并在刚毛发育中起作用。在本研究中，我们从N. lugens中克隆了一个apA （NlapA）和一个棘皮-鳞片同源物（NlASH）。若虫的NlapA和NlASH水平高于成虫，在若虫的胸部表达尤其高。2、4龄时，NlapA在大翼虫株系（MS）若虫中的表达高于短翼虫株系（BS）。在体内敲低NlapA和NlASH会在翅膀上产生类似的表型缺陷（失去刚毛、扭曲或直立的翅膀）。MS若虫NlapA基因的沉默导致成虫翅膀尺寸减小。此外，NlapA的缺失抑制了MS和BS中NlDl、Nlsal、Nlser、Nlwg和Nlwg的表达，但诱导了MS和BS中Nlubx和Nlnotch表达的差异反应。值得注意的是，NlapA可调节NlASH的表达。这些结果共同表明NlapA是机翼尺寸、刚毛形成和图案的上游调节剂。需要进一步研究dna -蛋白和蛋白-蛋白相互作用来阐明nlapa介导的翅膀发育调控。
Apterous A (apA), a member of the LIM-homeobox gene family, plays a critical role in the development of wing. The achaete-scute Complex (AS-C) encodes basic helix-loop-helix (bHLH) transcription factors and functions in bristle development. In the present study, we cloned apA (NlapA) and an achaete-scute homologue (NlASH) from N. lugens. Levels of NlapA and NlASH were higher in nymphs than adults, with particularly high expression in the thorax of nymphs. NlapA expressed more highly in nymphs of the macropterous strain (MS) than those of the brachypterous strain (BS) at 2nd and 4th instar. Knockdown of NlapA and NlASH in vivo generated similar phenotypic defects in the wing (loss-of-bristles, twisted or erect wing). Silencing of NlapA in nymphs of MS led to decreased wing size in adults. Moreover, depletion of NlapA suppressed expression of NlDl, Nlsal, Nlser, Nlvg and Nlwg, both in MS and BS, but induced differential responses of Nlubx and Nlnotch expression between MS and BS. Notably, expression of NlASH was regulated by NlapA. These results collectively indicate that NlapA is an upstream modulator of wing size, bristle formation and patterning. Further studies on DNA-protein and protein-protein interactions are required to elucidate NlapA-mediated regulation of wing development.
登录
查看更多内容
影响因子：
11.4
作者：
Fernández-Fúnez, P;Lu, CH;Botas, J
通讯作者：
Botas, J
影响因子：
16
作者：
Ramain, P;Khechumian, R;Heitzler, P
通讯作者：
Heitzler, P
影响因子：
4.8
作者：
Rajagopal, R;Sivakumar, S;Bhatnagar, RK
通讯作者：
Bhatnagar, RK
影响因子：
64.5
作者：
CAMPUZANO, S;BALCELLS, L;MODOLELL, J
通讯作者：
MODOLELL, J
影响因子：
56.9
作者：
Abouheif, E;Wray, GA
通讯作者：
Wray, GA