Transcriptomic and metabolomic profiling of melatonin treated soybean (Glycine max L.) under drought stress during grain filling period through regulation of secondary metabolite biosynthesis pathways.
Transcriptomic and metabolomic profiling of melatonin treated soybean (Glycine max L.) under drought stress during grain filling period through regulation of secondary metabolite biosynthesis pathways.
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通过调节次生代谢物生物合成途径，对灌浆期干旱胁迫下褪黑素处理的大豆 (Glycine max L.) 进行转录组学和代谢组学分析
DOI：
10.1371/journal.pone.0239701
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Cao L;Jin X;Zhang Y;Zhang M;Wang Y
提高大豆（Glycine max L.）在严重干旱条件下，以改善全球粮食安全状况。褪黑激素是植物体内普遍存在的一种激素，可以缓解干旱胁迫。早期，我们证明了外源褪黑激素处理可以增强干旱处理大豆的耐受性。然而，这种激素发挥抗旱性的潜在机制仍不清楚。本研究采用转录组学和代谢组学技术，以确定一些关键基因和途径调节褪黑激素对干旱条件的反应。结果表明，外源褪黑激素处理能提高干旱胁迫下叶片的相对含水量，降低电解质外渗率，提高种子产量。转录组学分析表明，大豆叶片中存在852个受干旱胁迫和褪黑激素调控的核心差异表达基因。最丰富的干旱响应基因主要参与“次生代谢物的生物合成”。干旱胁迫下的代谢谱分析表明，外源褪黑激素处理后，与耐旱性相关的次生代谢产物的积累水平较高。此外，我们强调的途径，包括苯丙素，类黄酮，甜菜碱，类固醇的生物合成途径，通过外源褪黑激素处理提高大豆耐旱性的重要作用。总之，本研究结果为褪黑激素作为抗旱剂在大豆栽培中的应用提供了详细的分子基础。
There is a growing need to enhance the productivity of soybean (Glycine max L.) under severe drought conditions in order to improve global food security status. Melatonin, a ubiquitous hormone, could alleviate drought stress in various plants. Earlier, we demonstrated that exogenous melatonin treatment could enhance the tolerance of drought-treated soybean. However, the underlying mechanisms by which this hormone exerts drought resistance is still unclear. The present study used transcriptomic and metabolomic techniques to determine some critical genes and pathways regulating melatonin response to drought conditions. Results showed that exogenous melatonin treatment could increase relative water content and decrease electrolyte leakage in the leaves and increase seed yield under drought stress. Transcriptomic analysis showed that there were 852 core differentially expressed genes (DEGs) that were regulated by drought stress and melatonin in soybean leaves. The most enriched drought-responsive genes are mainly involved in the ‘biosynthesis of secondary metabolites’. Metabolomic profiling under drought stress showed higher accumulation levels of secondary metabolites related to drought tolerance after exogenous melatonin treatment. Also, we highlighted the vital role of the pathways including phenylpropanoid, flavonoid, isoflavonoid, and steroid biosynthesis pathways for improvement of drought tolerance in soybean by exogenous melatonin treatment. In all, findings from this study give detailed molecular basis for the application of melatonin as a drought-resistant agent in soybean cultivation.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
--
作者：
Akpınar BA;Lucas SJ;Budak H
通讯作者：
Budak H
影响因子：
4.3
作者：
Hu, Wen-hai;Yan, Xiao-hong;Ogweno, Joshua Otieno
通讯作者：
Ogweno, Joshua Otieno
影响因子：
6.9
作者：
Li, Chao;Tan, Dun-Xian;Ma, Fengwang
通讯作者：
Ma, Fengwang
影响因子：
10.3
作者：
DUBBELS, R;REITER, RJ;SCHLOOT, W
通讯作者：
SCHLOOT, W