Development of a Thermoresponsive Valve Membrane for Microfluiic Paper-Based Analytical Devic
Development of a Thermoresponsive Valve Membrane for Microfluiic Paper-Based Analytical Devic
复制标题
用于微流控纸基分析装置的热响应阀膜的开发
DOI：
10.1109/transducers.2019.8808184
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
M. Miyazaki
中科院分区：
文献类型：
--
作者：
W. Iwasaki;N. Morita;Chiaki Sakurai;Y. Nakashima;Y. Nakanishi;M. Miyazaki
微流控纸基分析装置（μ PAD）因其体积小、易于制造、价格低廉、所需样品量少等优点，近年来受到研究人员和工业界的广泛关注。然而，由于流体控制手段有限，因此难以简化此类装置上的复杂测定。因此，在本研究中，我们开发了一个温敏阀膜聚合N-异丙基丙烯酰胺（NIPAAm）在多孔膜。PNIPAAm是一种温敏聚合物;在低于临界溶解温度（LCST; 32 °C）时，它变得亲水，而在高于临界溶解温度（LCST; 32 °C）时，它变得疏水。我们使用了绿色染料溶液，以调查流动的三维堆叠，T形纸网络包含的热敏阀。阀膜能够在25 °C下防止绿色染料通过（< LCST）。另一方面，绿色染料能够穿过阀膜和40 °C（> LCST）。结果表明，我们的温度响应阀可以用于µ PAD。此外，它有可能在µ PAD中自动实现复杂的免疫测定，并提高其通用性。
Microfluidic paper-based analytical devices (μPADs) have recently attracted the attention of researchers and industry because they are small, easily fabricated, inexpensive, and require small sample volumes. However, it is difficult to simplify complex assays on such devices because there are limited means of fluid control. Therefore, in the present study we developed a thermoresponsive valve membrane by polymerizing N-isopropylacrylamide (NIPAAm) in a porous membrane. PNIPAAm is a thermoresponsive polymer; it becomes hydrophilic below, and hydrophobic above, the lower critical solution temperature (LCST; 32 °C). We used a green dye solution to investigate flow in a three-dimensionally stacked, T-shaped paper network containing the thermoresponsive valve. The valve membrane was able to prevent the passage of green dye at 25 °C (< LCST). On the other hand, green dye was able to path through the valve membrane and 40 °C (> LCST). The result demonstrates that our thermoresponsive valve could be used in µPADs. Moreover, it has the potential to automatically enable complex immunoassays in µPADs and improve their versatility.
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影响因子：
6.1
作者：
Lutz BR;Trinh P;Ball C;Fu E;Yager P
通讯作者：
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影响因子：
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影响因子：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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